抗小鼠OCT4(OCT3)抗体,克隆40

抗人、鼠OCT4(OCT3)的小鼠单克隆IgG1抗体

产品号 #(选择产品)

产品号 #60059_C

抗人、鼠OCT4(OCT3)的小鼠单克隆IgG1抗体

总览

40抗体克隆可与OCT-3/4(又名POU5F1)发生反应。POU5F1是一种转录因子,对小鼠发育过程中内细胞团的形成以及未分化的小鼠和人类胚胎干细胞(ES)和诱导多能干细胞(iPS)的维持至关重要。它在未分化的小鼠和人类ES细胞、iPS细胞以及胚胎生殖细胞(EGCs)中高表达,并在分化过程中表达减少。它常用于评估多个物种的未分化ES细胞、iPS细胞和EGC的标记物。

亚型
一抗
 
靶抗原
OCT4 (OCT3)
 
别名
Oct3,OTF3,Oct4,OTF4,POU5F1
 
活性物种
人,小鼠
 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
多能干细胞
 
种属
人,小鼠
 
应用
流式细胞术,免疫荧光,Western印迹
 
研究领域
干细胞生物学
 
克隆
40
 
基因编号
18999 (mouse),5460 (human)
 
同种型
IgG1,kappa
 

Data Figures

(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by a goat antimouse IgG antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). (B) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1 isotype control antibody, followed by goat anti-mouse IgG, FITC.

Neurospheres derived from human fetal CNS tissue can be efficiently generated for multiple passages in NeuroCult™-ACF Proliferation Medium

(C) H1 cell nuclei stained with DAPI. (D) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60059
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60059
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human, Mouse
Clone 40
Gene Id 18999 (mouse), 5460 (human)
Alternative Names Oct3, OTF3, Oct4, OTF4, POU5F1
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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