产品号 #60059_C
Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
概述
The 40 antibody clone reacts with OCT-3/4, also known as POU5F1, a transcription factor that is essential for the formation of the inner cell mass during mouse development and for the maintenance of undifferentiated mouse and human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is expressed at high levels in undifferentiated mouse and human ES and iPS cells and in embryonic germ cells (EGCs), and exhibits decreased expression during differentiation. It is commonly used as a marker for the assessment of undifferentiated ES and iPS cells, and EGCs from multiple species.
Subtype
Primary Antibodies
Target Antigen
OCT4 (OCT3)
Alternative Names
Oct3, OTF3, Oct4, OTF4, POU5F1
Reactive Species
Human, Mouse
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Pluripotent Stem Cells
Species
Human, Mouse
Application
Flow Cytometry, Immunofluorescence, Western Blotting
Area of Interest
Stem Cell Biology
Clone
40
Gene ID
18999 (mouse), 5460 (human)
Isotype
IgG1, kappa
Data Figures
(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by a goat antimouse IgG antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). (B) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1 isotype control antibody, followed by goat anti-mouse IgG, FITC.
(C) H1 cell nuclei stained with DAPI. (D) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse OCT4 (OCT3) Antibody, Clone 40 Catalog #
60059 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse OCT4 (OCT3) Antibody, Clone 40 Catalog #
60059 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for PSC Quality Control
Resources and Publications
Educational Materials (3)
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