产品号 #18945
从小鼠肺组织、脾细胞或其他组织中分选CD45+白细胞的免疫磁珠正选
概述
使用EasySep™小鼠CD45正选试剂盒，通过免疫磁珠正选技术，从小鼠脾细胞、肺组织或其他组织样本中分离高纯度的小鼠CD45+细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。
在该EasySep™正选流程中，目的细胞被识别CD45的抗体复合物和磁珠标记。使用EasySep™磁极分离被标记的细胞，非目的细胞通过简单倾倒被去除，而目的细胞则被保留在管中。磁分选得到的目的小鼠CD45+细胞可立即用于流式细胞术、细胞培养及基于细胞的实验等下游应用。了解更多EasySep™免疫磁珠技术的工作原理。探索为您的实验流程优化的更多产品，包括培养基、补充、抗体等。
MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
SUBTYPE
Cell Isolation Kits
CELL TYPE
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Leukemia/Lymphoma Cells, Lymphocytes, Macrophages, Megakaryocytes, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Plasma, T Cells, T Cells, Other Subsets
SPECIES
Mouse
SAMPLE SOURCE
Lung, Other, Spleen
SELECTION METHOD
Positive
APPLICATION
Cell Isolation
BRAND
EasySep
AREA OF INTEREST
Immunology
实验数据
产品说明书及文档
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
应用领域
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
相关材料与文献
Educational Materials (10)
Publications (2)
HIF-1$\alpha$ modulates sex-specific Th17/Treg responses during hepatic amoebiasis. Journal of hepatology 2022 jan
Abstract
BACKGROUND & AIMS An invasive form of intestinal Entamoeba (E.) histolytica infection, which causes amoebic liver abscess, is more common in men than in women. Immunopathological mechanisms are responsible for the more severe outcome in males. Here, we used a mouse model of hepatic amoebiasis to investigate the contribution of hepatic hypoxia-inducible factor (HIF)-1$\alpha$ to T helper 17 (Th17)/regulatory T cell (Treg) responses in the context of the sex-specific outcome of liver damage. METHODS C57BL/6J mice were infected intrahepatically with E. histolytica trophozoites. HIF-1$\alpha$ expression was determined by qPCR, flow cytometry and immunohistochemistry. Tregs and Th17 cells were analysed by immunohistochemistry and flow cytometry. Finally, male and female hepatocyte-specific Hif1$\alpha$ knockout mice were generated, and the effect of HIF-1$\alpha$ on abscess development, the cytokine milieu, and Th17/Treg differentiation was examined. RESULTS E. histolytica infection increased hepatic HIF-1$\alpha$ levels, along with the elevated frequencies of hepatic Th17 and Treg cells. While the Th17 cell population was larger in male mice, Tregs characterised by increased expression of Foxp3 in female mice. Male mice displayed increased IL-6 expression, contributing to immunopathology; this increase in IL-6 expression declined upon deletion of hepatic HIF-1$\alpha$. In both sexes, hepatic deletion of HIF-1$\alpha$ reduced the Th17 cell frequency; however, the percentage of Tregs was reduced in female mice only. CONCLUSIONS Hepatic HIF-1$\alpha$ modulates the sex-specific outcome of murine E. histolytica infection. Our results suggest that in male mice, Th17 cells can be modulated by hepatic HIF-1$\alpha$ via IL-6, indicating marked involvement in the immunopathology underlying abscess development. Strong expression of Foxp3 by hepatic Tregs from female mice suggests a potent immunosuppressive function, leading to initiation of liver regeneration. LAY SUMMARY Infection with the parasite Entamoeba histolytica activates immunopathological mechanisms in male mice, which lead to liver abscesses that are larger than those in female mice. In the absence of the protein HIF-1$\alpha$ in hepatocytes, abscess formation is reduced; moreover, the sex difference in abscess size is abolished. These results suggest that HIF-1$\alpha$ modulates the immune response involved in the induction of immunopathology, resulting in differential disease susceptibility in males and females.
Human NK cells confer protection against HIV-1 infection in humanized mice. The Journal of clinical investigation 2022 dec
Abstract
The role of NK cells against HIV-1 infections remains to be elucidated in vivo. While humanized mouse models potentially could be used to directly evaluate human NK cell responses during HIV-1 infection, improved functional development of human NK cells in these hosts is needed. Here, we report the humanized MISTRG-6-15 mouse model, in which NK cells were quick to expand and exhibit degranulation, cytotoxicity, and proinflammatory cytokine production in nonlymphoid organs upon HIV-1 infection but had reduced functionality in lymphoid organs. Although HIV-1 infection induced functional impairment of NK cells, antiretroviral therapy reinvigorated NK cells in response to HIV-1 rebound after analytic treatment interruption. Moreover, a broadly neutralizing antibody, PGT121, enhanced NK cell function in vivo, consistent with antibody-dependent cellular cytotoxicity. Monoclonal antibody depletion of NK cells resulted in higher viral loads in multiple nonlymphoid organs. Overall, our results in humanized MISTRG-6-15 mice demonstrated that NK cells provided direct anti-HIV-1 responses in vivo but were limited in their responses in lymphoid organs.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
