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抗小鼠CD25抗体，克隆PC61.5

大鼠单克隆IgG1抗体，抗小鼠CD25
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产品号 #60009_C

大鼠单克隆IgG1抗体，抗小鼠CD25

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

PC61.5抗体识别小鼠CD25（低亲和力白细胞介素-2受体α链，IL-2Rα），CD25是一种~55 kDa的1型跨膜糖蛋白，在T细胞和B细胞祖细胞、活化的（非静息）T细胞和B细胞，调节性T细胞（Treg）细胞，树突状细胞以及活化的单核细胞和巨噬细胞上表达。CD25、CD4和FOXP3的共表达被认为是Treg细胞的表型特征。CD25本身对其IL-2配体具有低亲和力，但与CD122 （IL-2Rβ）和CD132 （IL-2Rγ）结合形成高亲和力的IL-2R受体。CD25是受体聚合和诱导淋巴细胞活化和增殖相关信号通路所必需的。PC61.5抗体结合可抑制IL-2与低亲和力和高亲和力受体的结合，从而可能抑制IL-2依赖性的增殖。PC61.5识别的表位不同于IL-2结合位点，也不同于抗体7D4和3C7识别的表位。

亚型
一抗
 
靶抗原
CD25
 
别名
IL-2受体亚基α，IL-2Ra，IL-2Ralpha，IL-2-RA，白细胞介素-2受体亚基α，Ly-43，p55，Tac
 
活性物种
小鼠
 
偶联
APC，Biotin 或 生物素，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
T 细胞，T 细胞，调节性细胞
 
种属
小鼠
 
应用
删除，流式细胞术，功能学筛选，免疫组化，免疫沉淀，中和及阻断
 
研究领域
免疫
 
克隆
PC61.5
 
基因编号
16184
 
同种型
IgG1，lambda
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071 (Catalog #60075), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled withRat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC (Catalog #60075AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE (Catalog #60075PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 4. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a rat IgG1 isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated rat IgG1 isotype control antibody followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ, 60009AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS, 60009PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, APC
Catalog #
60009AZ, 60009AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin
Catalog #
60009BT.2, 60009BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PE
Catalog #
60009PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5
Catalog #
60009PS, 60009PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD25 Antibody, Clone PC61.5
Catalog #
60009
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Mouse
Clone PC61.5
Gene Id 16184
Alternative Names IL-2 receptor subunit alpha, IL-2Ra, IL-2Ralpha, IL-2-RA, Interleukin-2 receptor subunit alpha, Ly-43, p55, Tac
Isotype IgG1, lambda
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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