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抗人SSEA-5抗体，克隆8e11

抗人SSEA-5小鼠单克隆IgG1抗体
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产品号 #60063_C

抗人SSEA-5小鼠单克隆IgG1抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

8e11抗体可与阶段特异性胚胎抗原5 (SSEA-5) 发生反应。SSEA-5是一种末端H型1聚糖(Fuc1-2Galβ1-3GlcNAcβ1)，在胚胎发生过程中表达于人类囊胚内细胞团细胞表面，也表达于未分化的人类胚胎干细胞(ES)和诱导多能干细胞(iPS)表面。SSEA-5的表达在细胞分化过程中迅速下调。8e11抗体可与其他多能性表面标志物（例如CD9和CD90，或CD50和CD200）联合使用，以从未完全分化的干细胞培养物中分选并去除未分化的畸胎瘤形成细胞。

亚型
一抗
 
靶抗原
SSEA-5
 
别名
阶段特异性胚胎抗原-5
 
活性物种
人
 
偶联
Alexa Fluor 488，APC，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
多能干细胞
 
种属
人
 
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
干细胞生物学
 
克隆
8e11
 
基因编号
Not applicable
 
同种型
IgG1，kappa
 

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Data Figures

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human embryonic stem (ES) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody.
(D) Human iPS cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC. (C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, FITC
Catalog #
60063FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11
Catalog #
60063.1, 60063
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, PE
Catalog #
60063PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, APC
Catalog #
60063AZ, 60063AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, FITC
Catalog #
60063FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11
Catalog #
60063.1, 60063
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, PE
Catalog #
60063PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human SSEA-5 Antibody, Clone 8e11, APC
Catalog #
60063AZ, 60063AZ.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Clone 8e11
Gene Id Not applicable
Alternative Names Stage-specific embryonic antigen-5
Isotype IgG1, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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