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抗人MUC1（CD227）抗体，克隆16A

抗人MUC1（CD227）的小鼠单克隆IgG1抗体
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产品号 #60155_C

抗人MUC1（CD227）的小鼠单克隆IgG1抗体

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产品说明书及文档
应用领域
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总览

16A抗体靶向人源MUC1，一种分子量>250 kDa的高度糖基化I型跨膜蛋白，在大多数腺体及导管上皮细胞以及多种造血细胞的表面表达。MUC1糖蛋白的一个特征是其核心结构域由可变数量的串联重复序列和多个寡糖侧链组成。由于MUC1的胞外结构域长度显著超过多数膜蛋白，因此它被认为可能在细胞间粘附以及细胞-基质粘附中发挥作用。该蛋白在大多数人腺癌中高表达，并且与不良预后相关。MUC1在乳腺组织中特异性分布于腔上皮细胞顶质膜。克隆 16A 抗体对糖基化形式的MUC1具有更高的亲和力。

亚型
一抗
 
靶抗原
MUC1 (CD227)
 
别名
CD227，EMA，Episialin，上皮膜抗原，HMFG抗原，MAM6，黏蛋白1，PEM，多态性上皮黏蛋白
 
活性物种
人
 
偶联
APC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
气道细胞
 
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化
 
研究领域
上皮细胞研究
 
克隆
16A
 
基因编号
4582
 
同种型
IgG1，lambda
 

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Data Figures

(A) Primary human airway epithelial cells were cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface, then cryo-sectioned and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and an anti-human NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (B) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by an anti-mouse IgG1 antibody, PE and anti-human CD3 antibody, Clone HIT3a, Pacific Blue™. (C) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by an anti-mouse IgG1 antibody, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

(A) Flow cytometry analysis of human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface. Cells were enzymatically dissociated and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram). (C) Flow cytometry analysis human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE and anti-human CD3 antibody, clone HIT3a, Pacific Blue™. (D) Flow cytometry analysis of PHA-activated human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

(A) Flow cytometry analysis of human airway epithelial cells cultured in PneumaCult™-ALI Medium at the air-liquid interface. Cells were enzymatically dissociated and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ, solid line histogram). (B) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with phytohemagglutinin (PHA) for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC (Catalog #60011FI). (C) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A, APC
Catalog #
60155AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE
Catalog #
60155PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A
Catalog #
60155
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A, APC
Catalog #
60155AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE
Catalog #
60155PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human MUC1 (CD227) Antibody, Clone 16A
Catalog #
60155
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (1)

Pulmonary Research
Brochure
Pulmonary Research

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更多信息
Clone 16A
Gene Id 4582
Alternative Names CD227, EMA, Episialin, Epithelial membrane antigen, HMFG antigen, MAM6, Mucin 1, PEM, Polymorphic epithelial mucin
Isotype IgG1, lambda
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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