JavaScript 似乎在您的浏览器中被禁用。 为了您在我们网站能获得最好的体验，建议在浏览器中打开Javascript。

Anti-Human CD80 (B7-1) Antibody, Clone 2D10.4

Mouse monoclonal antibody against human, rhesus CD80 (B7-1)

率先评论此产品

产品号 #100-1599_C

Mouse monoclonal antibody against human, rhesus CD80 (B7-1)

跳到结尾的图片库

Anti-Human CD80 (B7-1) Antibody, Clone 2D10.4

跳转到图像库的开头

概述

实验数据

产品说明书及文档

概述

This monoclonal antibody (clone 2D10.4) reacts with human cluster of differentiation 80 (CD80), also known as B7-1, a 60 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed by activated B cells, T cells, macrophages, and dendritic cells. Use this recombinant monoclonal antibody in applications requiring detection of CD80, such as flow cytometry (FC). This antibody clone has been verified for labeling CD80+ primary human M1 macrophages grown in ImmunoCult™-SF Macrophage Medium (Catalog #10961) containing Human Recombinant M-CSF (Catalog #78057), and then polarized with Human Recombinant IFN-gamma (Catalog #78020) and Lipopolysaccharide from E. coli (O55:B5) (Catalog #100-1270).
CD80 has a high affinity for binding to two T cell receptors; CD28 and CD152 (also known as CTLA-4). The interaction of CD80 with CD28 provides a potent co-stimulatory signal for T cell activation through the CD3 complex, while its interaction with CD152 provides an inhibitory signal for T cell activation. CD80 together with CD86, another ligand of CD28 also known as B7-2, plays a major role in regulation of T cell activation. CD80 dysfunction is associated with diseases such as squamous cell carcinoma of gallbladder (SCC) and myocarditis. This antibody can also be used as a marker to assess classically activated type 1 (M1) macrophages.

Subtype
Primary Antibodies

Target Antigen
CD80 (B7-1)

Alternative Names
B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Reactive Species
Human, Rhesus

Conjugation
APC, PE

Host Species
Mouse

Cell Type
B Cells, Dendritic Cells, Macrophages, T Cells

Species
Human

Application
Flow Cytometry

Area of Interest
Immunology

Clone
2D10.4

Gene ID
941

Isotype
IgG1, kappa

Data Figures

Figure 1. Flow Cytometry Data for Monocyte-Derived Macrophages Labeled with Anti-Human CD80 Antibody

Flow cytometry analysis of primary human monocyte-derived macrophages polarized toward M1 (solid line histogram; purple) or M2a (solid line histogram; gold) subtypes cultured using ImmunoCult™-SF Macrophage Medium and labeled with Anti-Human CD80 Antibody, Clone 2D10.4, PE. Labeling of human monocyte-derived M1 macrophages with a mouse IgG1, kappa, PE isotype control antibody (Catalog #60070PE) is shown (filled histogram; grey).