抗人CD68 抗体,克隆Y1/82A

小鼠单克隆IgG2b抗体,抗人CD68

产品号 #(选择产品)

产品号 #60105_C

小鼠单克隆IgG2b抗体,抗人CD68

总览

Y1/82A抗体识别人CD68,CD68是一种~110 kDa的1型跨膜糖蛋白,是唾液粘蛋白、LAMP和清道夫受体家族的成员。CD68的重度糖基化胞外结构域,包含一个富含脯氨酸的间隔区,将粘蛋白和LAMP结构域分开,可与低密度脂蛋白及某些凝集素和选择素结合,但 CD68 的功能仍不清楚。CD68在溶酶体、核内体和细胞质颗粒中高表达,在巨噬细胞、单核细胞、中性粒细胞、嗜碱性细胞、树突状细胞和NK细胞表面表达较弱。在γ/δ T细胞、LAK细胞、成纤维细胞、内皮细胞、B细胞亚群和造血祖细胞以及各种非造血组织(如肝和肾)的细胞质中也能检测到它。Y1/82A抗体识别的表位不同于抗体克隆Y2/131、EBM11、Ki-M6和KP1。据报道,与KP1相比,Y1/82A对单核细胞和巨噬细胞更具特异性。

亚型
一抗
 
靶抗原
CD68
 
别名
GP110,LAMP4,溶酶体相关膜蛋白,Macrosialin,SCARD1,清道夫受体D类成员1
 
活性物种

 
偶联
Alexa Fluor 488,APC,Biotin 或 生物素,FITC,PE,未偶联的
 
宿主物种
小鼠
 
细胞类型
粒细胞及其亚群,髓系细胞
 
种属

 
应用
流式细胞术,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,Western印迹
 
研究领域
免疫
 
克隆
Y1/82A
 
基因编号
968
 
同种型
IgG2b,kappa
 

Data Figures

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, followed by anti-hamster (Armenian) IgG, FITC (filled histogram) or an Armenian hamster IgG isotype control antibody followed by anti-hamster (Armenian) IgG, FITC (open histogram).

Figure 2.Data for Alexa Fluor® 488-conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488 (filled histogram) or an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 3. Data for APC-conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (filled histogram) or an Armenian hamster IgG APC isotype control antibody (solid line histogram).

Figure 4. Data for Biotin-conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin followed by streptavidin (SAV) APC (filled histogram) or an Armenian hamster IgG biotin isotype control antibody followed by SAV APC (solid line histogram).

Figure 5. Data for FITC-conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC (filled histogram) or an Armenian hamster IgG FITC isotype control antibody (solid line histogram).

Figure 6. Data for PE-conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (filled histogram) or an Armenian hamster IgG PE isotype control antibody (solid line histogram).

Figure 7. Data for PerCP-Cy5.5 Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5 (filled histogram) or an Armenian hamster IgG isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60105AD, 60105AD.1
Lot #
All
Language
English
Catalog #
60105AZ
Lot #
All
Language
English
Catalog #
60105BT
Lot #
All
Language
English
Catalog #
60105FI
Lot #
All
Language
English
Catalog #
60105, 60105.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60105AD, 60105AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60105AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60105BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60105FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60105, 60105.1
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)

更多信息

更多信息
Species Human
Clone Y1/82A
Gene Id 968
Alternative Names GP110, LAMP4, Lysosomal-associated membrane protein, Macrosialin, SCARD1, Scavenger receptor class D member 1
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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