抗人CD33抗体,克隆HIM3-4

小鼠单克隆IgG1抗体,抗人、黑猩猩CD33

产品号 #(选择产品)

产品号 #60096_C

小鼠单克隆IgG1抗体,抗人、黑猩猩CD33

总览

HIM3-4抗体识别CD33(Siglec3),CD33是~67 - 75 kDa的1型跨膜唾液粘附蛋白,属于免疫球蛋白超家族。CD33在髓系定向祖细胞、单核细胞、粒细胞、树突状细胞、肥大细胞、驻留巨噬细胞、B细胞的亚群以及活化的T细胞和NK细胞上表达。CD33是用于将髓系白血病和淋系白血病或红系白血病区分开来的主要标志物。在外周血中,CD33在单核细胞上高水平表达,在粒细胞上低水平表达。CD33作为唾液酸依赖的细胞粘附分子,介导细胞间的蛋白-聚糖和蛋白-蛋白相互作用。CD33也被认为在免疫反应过程中发挥抑制受体的作用。据报导,HIM3-4结合CD33的细胞外C2 Ig结构域,并且不阻断其他几种抗CD33抗体克隆(包括P67.6、WM53和WM54)的后续结合。CD33已被鉴定出两种亚型CD33M和CD33m,在上述克隆中只有HIM3-4能够同时识别这两种亚型。

亚型
一抗
 
靶抗原
CD33
 
别名
gp67,p67,Sialic acid-binding immunoglobulin-like lectin 3,SIGLEC3,Siglec-3
 
活性物种
Chimpanzee,人
 
偶联
FITC,PE,未偶联的
 
宿主物种
小鼠
 
细胞类型
粒细胞及其亚群,髓系细胞
 
种属
人,非人灵长类
 
应用
流式细胞术,免疫细胞化学,免疫荧光
 
研究领域
免疫
 
克隆
HIM3-4
 
基因编号
945
 
同种型
IgG1,kappa
 

Data Figures

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse Ig1, kappa FITC isotype control antibody is shown (open histogram).

Data for Unconjugated

Figure 2. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD33 Antibody, Clone HIM3-4, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60096PE.1, 60096PE
Lot #
All
Language
English
Catalog #
60096FI.1, 60096FI
Lot #
All
Language
English
Catalog #
60096
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60096PE.1, 60096PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60096FI.1, 60096FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60096
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (3)

更多信息

更多信息
Species Human, Non-Human Primate
Clone HIM3-4
Gene Id 945
Alternative Names gp67, p67, Sialic acid-binding immunoglobulin-like lectin 3, SIGLEC3, Siglec-3
Isotype IgG1, kappa
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