产品号 #60136_C
小鼠单克隆IgG1抗体,抗人CD326 (EpCAM)
VU-1D9抗体识别人上皮细胞黏附分子(EpCAM或CD326),这是一种约40 kDa的I型跨膜糖蛋白,作为一种同源性、Ca²⁺ 非依赖性的黏附分子发挥作用。VU-1D9抗体的结合依赖于EpCAM的细胞外EGF-1结构域的存在。EpCAM 参与多种细胞过程,包括信号转导、迁移、增殖和分化。其在红系生成早期即被表达,并被鉴定为一种干细胞标志物。EpCAM 蛋白主要位于大多数正常上皮细胞的基侧膜,在骨髓、结肠和肺组织中表达较高。由于 EpCAM 不在间皮细胞或间皮瘤中表达,因此被广泛用作区分间皮瘤与癌细胞的诊断标志物,并作为癌症免疫治疗的靶点。VU-1D9 抗体还被用于识别和分离循环肿瘤细胞(CTCs)。
亚型
一抗
靶抗原
CD326 (EpCAM)
别名
EGP2,EpCAM,上皮细胞黏附分子,ESA,TACSTD1,TROP-1
活性物种
人
偶联
未偶联的
宿主物种
小鼠
细胞类型
乳腺细胞
种属
人
应用
细胞分选,流式细胞术,免疫组化
研究领域
上皮细胞研究,干细胞生物学
克隆
VU-1D9
基因编号
4072
同种型
IgG1,kappa
Figure 1. Data for Unconjugated
Flow cytometry analysis of human MCF7 cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).
Figure 2. Data for FITC-conjugated
(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of human MCF7 cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).
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Species | Human |
---|---|
Clone | VU-1D9 |
Gene Id | 4072 |
Alternative Names | EGP2, EpCAM, Epithelial cell adhesion molecule, ESA, TACSTD1, TROP-1 |
Isotype | IgG1, kappa |
用于人乳腺球和肿瘤球的培养
小鼠单克隆IgG1, kappa同型对照抗体
小鼠单克隆IgG1抗体,抗人MUC1 (CD227)
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