产品号 #60136_C
Mouse monoclonal IgG1 antibody against human CD326 (EpCAM)
概述
The VU-1D9 antibody reacts with the human epithelial cell adhesion molecule (EpCAM or CD326), an ~40 kDa type I transmembrane glycoprotein, which functions as a homophilic and Ca++-independent adhesion molecule. Binding of the VU-1D9 antibody requires the presence of the extracellular EGF-1 domain of EpCAM. EpCAM has roles in several cellular processes, including signaling, migration, proliferation, and differentiation. It is expressed early during erythrogenesis and has been identified as a stem cell marker. The EpCAM protein is found on the basolateral membrane of most normal epithelial cells and is highly expressed in bone marrow, colon, and lung. EpCAM is not expressed on mesothelial cells or mesotheliomas, and so is widely used as a diagnostic marker to distinguish mesothelioma and carcinoma cells, as well as serving as a target for immunotherapeutic treatment of carcinomas. The VU-1D9 antibody has been used to identify and isolate circulating tumor cells.
Subtype
Primary Antibodies
Target Antigen
CD326 (EpCAM)
Alternative Names
EGP2, EpCAM, Epithelial cell adhesion molecule, ESA, TACSTD1, TROP-1
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Mammary Cells
Species
Human
Application
Cell Isolation, Flow Cytometry, Immunohistochemistry
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
VU-1D9
Gene ID
4072
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human MCF7 cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).
Figure 2. Data for FITC-conjugated
(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of human MCF7 cells labeled with Anti-Human CD326 (EpCAM) Antibody, Clone VU-1D9, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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