产品号 #60135_C
小鼠单克隆IgG2b抗体,抗人、恒河猴、食蟹猴CD32
FLI8.26抗体识别人CD32 (FcγRII),CD32是一种~40 kDa的1型跨膜糖蛋白,介导多种功能,包括吞噬、细胞毒性、免疫调节和血小板聚集。FLI8.26抗体与单核细胞、粒细胞和血小板存在交叉反应。CD32由3个基因(A、B、C)编码,通过mRNA的选择性剪接产生至少6个异构体,即IIa1、IIa2、IIb1、IIb2、IIb3和IIc。单核/巨噬细胞、胎盘滋养细胞和内皮细胞表达所有亚型。此外,B细胞表达IIb亚型;血小板、粒细胞表达IIa亚型,B细胞弱表达IIa亚型。NK细胞和中性粒细胞表达IIc亚型。克隆FLI8.26已被证实能结合FcγRIIa和FcγRIIb,据报导也能结合FcγRIIc,而FcγRIIc在信号肽、胞外和跨膜结构域与FcγRIIb具有相同的氨基酸序列。克隆FLI8.26可抑制FcγRIIa特异性抗体克隆IV.3的结合。CD32与IgG单体的Fc段结合较弱,但与IgG聚集体和免疫复合物结合较强。这些相互作用可导致基于抗体的检测和细胞分选实验中的非特异性标记,而FLI8.26抗体可以作为阻断抗体来减少非特异性结合。
亚型
一抗
靶抗原
CD32
别名
FCR II,FcγRII
活性物种
Baboon,Chimpanzee,Cynomolgus,人,Rhesus
偶联
未偶联的
宿主物种
小鼠
细胞类型
B 细胞,粒细胞及其亚群,单核细胞
种属
人,非人灵长类
应用
细胞分选,流式细胞术
研究领域
免疫
克隆
FLI8.26
基因编号
2212
同种型
IgG2b,kappa
Figure 1. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, followed by a rat anti-mouse IgG2b antibody, PE (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by a rat anti-mouse IgG2b antibody, PE (solid line histogram).
Figure 2. Data for FITC-conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, FITC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI; solid line histogram).
Figure 3. Data for PE-conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, PE (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE; solid line histogram).
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Species | Human, Non-Human Primate |
---|---|
Clone | FLI8.26 |
Gene Id | 2212 |
Alternative Names | FCR II, FcγRII |
Isotype | IgG2b, kappa |
通过免疫磁珠负选分离出无磁珠标记的人T细胞
免疫磁珠负选人B细胞
小鼠单克隆IgG2b抗体,抗人CD32
小鼠单克隆IgG2b, kappa同型对照抗体
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