产品号 #60135_C
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD32
概述
The FLI8.26 antibody reacts with human CD32 (FcγRII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation, and platelet aggregation. The FLI8.26 antibody cross-reacts with monocytes, granulocytes, and platelets. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes/macrophages, placental trophoblasts, and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. Clone FLI8.26 has been verified to bind to FcγRIIa and FcγRIIb, and reportedly binds to FcγRIIc, which shares an identical amino acid sequence to FcγRIIb in the signal peptide, extracellular and transmembrane domains. Clone FLI8.26 inhibits binding of the FcγRIIa-specific clone IV.3 antibody in flow cytometry analyses. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the FLI8.26 antibody may be used as a blocking antibody to reduce non-specific binding.
Subtype
Primary Antibodies
Target Antigen
CD32
Alternative Names
FCR II, FcγRII
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Rhesus
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
B Cells, Granulocytes and Subsets, Monocytes
Species
Human, Non-Human Primate
Application
Cell Isolation, Flow Cytometry
Area of Interest
Immunology
Clone
FLI8.26
Gene ID
2212
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, followed by a rat anti-mouse IgG2b antibody, PE (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by a rat anti-mouse IgG2b antibody, PE (solid line histogram).
Figure 2. Data for FITC-conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, FITC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI; solid line histogram).
Figure 3. Data for PE-conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD32 Antibody, Clone FLI8.26, PE (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE; solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26 Catalog #
60135 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26, PE Catalog #
60135PE Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26, FITC Catalog #
60135FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26 Catalog #
60135 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26, PE Catalog #
60135PE Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD32 Antibody, Clone FLI8.26, FITC Catalog #
60135FI.1 Lot #
All Language
English Resources and Publications
Educational Materials (2)
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