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抗人CD138（Syndecan-1）抗体，克隆 MI15

抗人、恒河猴、食蟹猴CD138（syndecan-1）的小鼠单克隆IgG1抗体
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产品号 #60003_C

抗人、恒河猴、食蟹猴CD138（syndecan-1）的小鼠单克隆IgG1抗体

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总览

MI15抗体与细胞外表位CD138 （Syndecan-1）反应，CD138 （Syndecan-1）是一种分子量~85 - 95 kDa的1型跨膜糖蛋白，表达于前B细胞、未成熟B细胞、正常和恶性浆细胞（但不表达于成熟循环B细胞）以及非造血细胞（如胚胎间充质细胞、内皮细胞、上皮细胞和神经细胞）的表面。CD138的表达被用作几多种肿瘤的诊断标志物。CD138主要作为一种受体，通过连接细胞外基质和细胞骨架来调节细胞增殖、细胞迁移和细胞-基质关联。附着在CD138上的硫酸肝素和硫酸软骨素部分与多种蛋白质相关联，包括胶原蛋白、纤维连接蛋白、肌腱蛋白、血小板反应蛋白和特定细胞因子。MI15抗体识别与克隆DL-101抗CD138抗体不同的表位，但阻断克隆B-B4的结合。

亚型
一抗
 
靶抗原
CD138 (Syndecan-1)
 
别名
B-B4，SDC1，突触黏附素‑1
 
活性物种
Cynomolgus，人，Rhesus
 
偶联
APC，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
B 细胞，血浆
 
种属
人，非人灵长类
 
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
癌症，免疫
 
克隆
MI15
 
基因编号
6382
 
同种型
IgG1，kappa
 

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Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) is shown (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) is shown (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) is shown (solid line histogram).

Data for Unconjugated

Figure 4. Data for Unconjugated

Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (open histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC
Catalog #
60003FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, PE
Catalog #
60003PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE
Catalog #
60003PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, FITC
Catalog #
60003FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15
Catalog #
60003
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, APC
Catalog #
60003AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC
Catalog #
60003AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC
Catalog #
60003FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, PE
Catalog #
60003PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE
Catalog #
60003PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, FITC
Catalog #
60003FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15
Catalog #
60003
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan­1) Antibody, Clone MI15, APC
Catalog #
60003AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC
Catalog #
60003AZ.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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更多信息
Species Human, Non-Human Primate
Clone MI15
Gene Id 6382
Alternative Names B-B4, SDC1, syndecan-1
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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