产品号 #60003_C
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD138 (syndecan-1)
概述
The MI15 antibody reacts with an extracellular epitope on CD138 (Syndecan-1), an ~85 - 95 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells), as well as on non-hematopoietic cells such as embryonic mesenchymal cells, endothelial, epithelial and neural cells. CD138 expression is used as a diagnostic marker for several types of tumors. CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. Heparin sulfate and chondroitin sulfate moieties attached to CD138 associate with several proteins, including collagens, fibronectin, tenascin, thrombospondin and certain cytokines. The MI15 antibody recognizes a different epitope than that of the clone DL-101 anti-CD138 antibody but blocks binding of clone B-B4.
Subtype
Primary Antibodies
Target Antigen
CD138 (Syndecan-1)
Alternative Names
B-B4, SDC1, syndecan-1
Reactive Species
Cynomolgus, Human, Rhesus
Conjugation
APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells, Plasma
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Cancer, Immunology
Clone
MI15
Gene ID
6382
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for APC-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) is shown (solid line histogram).
Figure 2. Data for FITC-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) is shown (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) is shown (solid line histogram).
Figure 4. Data for Unconjugated
Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (open histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15 Catalog #
60003 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15 Catalog #
60003 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Total Lymphocyte Research
Resources and Publications
Educational Materials (3)
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