STEMdiff™ 神经诱导培养基

用于人 ES 和 iPS 细胞神经诱导的成分明确的无血清培养基

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产品号 #（选择产品）

产品号 #05835_C

用于人 ES 和 iPS 细胞神经诱导的成分明确的无血清培养基

产品优势

STEMdiff™ 神经诱导培养基，250 mL（目录号 #05835）
STEMdiff™ 神经诱导培养基，2 x 250 mL（目录号 #05839）

产品组分包括

成分明确，无血清
快速高效的神经诱导
兼容拟胚体和单层培养方案
可重复分化多种在mTeSR™ Plus、mTeSR™ 1或TeSR™-AOF中维持的ES细胞和iPS细胞系
方便、便捷、用户友好的格式和操作流程

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总览

STEMdiff™ 神经诱导培养基是一种成分明确的无血清培养基，用于诱导人类胚胎干细胞 (ES) 和诱导性多能干细胞 (iPS) 的神经分化。该培养基能够通过基于胚状体或单层培养的方案高效生成神经祖细胞。

您可以在我们的按需神经诱导课程中学习如何从人类多能干细胞 (hPSC) 生成神经祖细胞，并浏览我们关于使用胚状体法或单层法进行 hPSC 神经诱导的技术技巧。

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

STEMdiff™ Neural Induction Medium

Catalog #

05839, 05835

Lot #

All

Language

English

Document Type

Technical Manual

Product Name

STEMdiff™ Neural Induction Medium

Catalog #

05835

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

STEMdiff™ Neural Induction Medium

Catalog #

05839, 05835

Lot #

All

Language

English

Publications (37)

A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues. M. Robinson et al. Biosensors 2019 apr

Abstract

We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Neurons require soft substrates to differentiate and mature, however measuring the elastic moduli of soft substrates remains difficult to accurately measure using standard protocols such as atomic force microscopy or shear rheology. Here we validate a direct method for acquiring elastic modulus of fibrin using a modified Hertz model for thin films. In this method, spherical indenters are positioned on top of the fibrin samples, generating an indentation depth that is then correlated with elastic modulus. Neurons function by transmitting electrical signals to one another and being able to assess the development of electrical signaling serves is an important verification step when engineering neural tissues. We then validated a protocol wherein the electrical activity of motor neural cultures is measured directly by a voltage sensitive dye and a microplate reader without causing damage to the cells. These protocols provide a non-destructive method for characterizing the mechanical and electrical properties of living spinal cord tissues using novel biosensing methods.

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Comparative characterization of human induced pluripotent stem cells (hiPSC) derived from patients with schizophrenia and autism. L.-M. Grunwald et al. Translational psychiatry 2019

Abstract

Human induced pluripotent stem cells (hiPSC) provide an attractive tool to study disease mechanisms of neurodevelopmental disorders such as schizophrenia. A pertinent problem is the development of hiPSC-based assays to discriminate schizophrenia (SZ) from autism spectrum disorder (ASD) models. Healthy control individuals as well as patients with SZ and ASD were examined by a panel of diagnostic tests. Subsequently, skin biopsies were taken for the generation, differentiation, and testing of hiPSC-derived neurons from all individuals. SZ and ASD neurons share a reduced capacity for cortical differentiation as shown by quantitative analysis of the synaptic marker PSD95 and neurite outgrowth. By contrast, pattern analysis of calcium signals turned out to discriminate among healthy control, schizophrenia, and autism samples. Schizophrenia neurons displayed decreased peak frequency accompanied by increased peak areas, while autism neurons showed a slight decrease in peak amplitudes. For further analysis of the schizophrenia phenotype, transcriptome analyses revealed a clear discrimination among schizophrenia, autism, and healthy controls based on differentially expressed genes. However, considerable differences were still evident among schizophrenia patients under inspection. For one individual with schizophrenia, expression analysis revealed deregulation of genes associated with the major histocompatibility complex class II (MHC class II) presentation pathway. Interestingly, antipsychotic treatment of healthy control neurons also increased MHC class II expression. In conclusion, transcriptome analysis combined with pattern analysis of calcium signals appeared as a tool to discriminate between SZ and ASD phenotypes in vitro.

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Multiplication of the SNCA locus exacerbates neuronal nuclear aging. L. Tagliafierro et al. Human molecular genetics 2019

Abstract

Human-induced Pluripotent Stem Cell (hiPSC)-derived models have advanced the study of neurodegenerative diseases, including Parkinson's disease (PD). While age is the strongest risk factor for these disorders, hiPSC-derived models represent rejuvenated neurons. We developed hiPSC-derived Aged dopaminergic and cholinergic neurons to model PD and related synucleinopathies. Our new method induces aging through a `semi-natural' process, by passaging multiple times at the Neural Precursor Cell stage, prior to final differentiation. Characterization of isogenic hiPSC-derived neurons using heterochromatin and nuclear envelope markers, as well as DNA damage and global DNA methylation, validated our age-inducing method. Next, we compared neurons derived from a patient with SNCA-triplication (SNCA-Tri) and a Control. The SNCA-Tri neurons displayed exacerbated nuclear aging, showing advanced aging signatures already at the Juvenile stage. Noteworthy, the Aged SNCA-Tri neurons showed more $\alpha$-synuclein aggregates per cell versus the Juvenile. We suggest a link between the effects of aging and SNCA overexpression on neuronal nuclear architecture.

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