产品号 #60147_C
Mouse monoclonal IgG1 antibody against human epithelial cells
概述
The 5E11.3.1 antibody reacts with an unidentified marker expressed on the surface of most luminal cells of normal human mammary epithelium. Weak labeling (or no labeling) is observed within the basal cell compartment of the mammary epithelium. The 5E11.3.1 antibody gives variable labeling on malignant breast epithelium. Positive labeling is observed on the following cell lines: MCF7, CAMA, T47D, BT-10, BT-20, COLO 205, and WiDr. Labeling is negative on human bone marrow cells, blood cells, lymphoid and mesenchymal tissues, mammary and marrow fibroblast cultures, and human embryonic stem cell line H9.
Subtype
Primary Antibodies
Target Antigen
Human Epithelial Cell
Alternative Names
Not applicable
Reactive Species
Human
Conjugation
FITC, Unconjugated
Host Species
Mouse
Cell Type
Mammary Cells
Species
Human
Application
Cell Isolation, Flow Cytometry, Immunohistochemistry
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
5E11.3.1
Gene ID
4072
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for FITC-conjugated
Flow cytometry analysis of human MCF7 cells labeled with Anti-Human Epithelial Cell Antibody, Clone 5E11.3.1, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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