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StemSpan™ NK Cell Differentiation Supplement (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes
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  • Promotes the production of hundreds of CD14+ monocytes per input CD34+ human CB cell in 14-day liquid cultures.

  • Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium.

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD14+ monocytes from CD34+ cells isolated from human cord blood (CB) samples.

    StemSpan™ Myeloid Expansion Supplement II (100X) is intended for use in combination with any of the following StemSpan™ media:
    • StemSpan™ SFEM (Catalog #09600)
    • StemSpan™ SFEM II (Catalog #09605)
    • StemSpan™-XF (Catalog #100-0073)
    • StemSpan™-AOF (Catalog #100-0130)

    When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.

    Data Figures

    Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II

    Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    StemSpan™ Myeloid Expansion Supplement II (100X)
    Catalog #
    02694
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Product Name
    StemSpan™ Myeloid Expansion Supplement II (100X)
    Catalog #
    02694
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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