切换导航
  • 比较产品
我的购物车

STEMdiff™带分支结构的肺类器官试剂盒

用于hPSC来源分支肺类器官成熟的无血清培养基
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #100-0528_C

用于hPSC来源分支肺类器官成熟的无血清培养基

产品组分包括

  • STEMdiff™肺基础培养基，180ml
  • STEMdiff™肺补充液（10X）， 20ml
  • STEMdiff™分支肺类器官补充B (100X), 1.75 mL
main product photo
Interested in trying STEMCELL's organoid products for respiratory research? Fill out this form to request information about introductory offers.
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. Costar® 24-Well Flat-Bottom Plate, Tissue Culture-Treated
    Costar® 24-Well Flat-Bottom Plate, Tissue Cu...

    Clear polystyrene flat-bottom, tissue culture-treated multiwell cell culture plate with lid

  4. mTeSR™1
    mTeSR™1

    cGMP, feeder-free maintenance medium for human ES and iPS cells

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

使用STEMdiff™分支肺类器官成熟试剂盒，在长期（>28天）培养中成熟和维持人类分支肺类器官。

无血清STEMdiff™分支肺类器官成熟试剂盒可用于再生成熟的人胚胎干（ES）和诱导多能干（iPS）细胞衍生的肺芽类器官STEMdiff™分支肺类器官试剂盒。由此产生的类器官将形成近端和远端样分支气道上皮结构，表达EPCAM、NKX2.1、SOX2、SOX9、MUC1和P63。当在Matrigel®三明治中保存超过28天时，更成熟的肺细胞标志物（如SFTPC， SFTPB和ABCA3）的表达水平增加。

有关使用STEMdiff™分支肺类器官培养基系统启动和成熟分支肺类器官的详细信息，请参阅产品信息表(PIS).

Subtype
Specialized Media
 
Cell Type
Epithelial Cells, PSC-Derived, Mesenchymal Cells, PSC-Derived, Pluripotent Stem Cells
 
Species
Human
 
Application
Differentiation, Genome Editing, Organoid Culture
 
Brand
STEMdiff
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Infectious Diseases, Respiratory Research, Transplantation Research
 
Formulation Category
Serum-Free
 

查看更多 显示更少

Data Figures

Schematic showing the different stages in the STEMdiff™ Branching Lung Organoid Kit protocol and the representative morpholgy at each stage.

Figure 1. Generation Of Branching Lung Organoids Using the STEMdiff™ Branching Lung Organoid Kit

(A) Human PSC cultures progress through a four-stage differentiation process to generate human branching lung organoids. By the end of stage 1 (day 3), cultures exhibit characteristics typical of definitive endoderm and anterior foregut differentiation is initiated. During stage 2 (days 3 - 6) anterior foregut endoderm buds are released from the monolayer, and are then suspended to form ventralized lung bud organoids in stage 3 (days 6 - 14). In stage 4, the lung bud organoids are embedded into Matrigel sandwich cultures to mature into branching lung organoids. (B) Morphological representation of the culture at different stages.

Flow cytometry data at the end of stage 2 demonstrating expression of SOX2 and absence of CDX2 in AFE buds and brnaching lung organoids cultured beyond stage 4 demonstrating the expression of CPM and NKX2.1.

Figure 2. STEMdiff™ Branching Lung Organoid Kit Supports Expression of Key Lung Markers at Different Stages

Flow cytometric analysis at different stages of the STEMdiff™ Branching Lung Organoid Kit workflow shows protein expression of key lung markers. (A) Anterior foregut endoderm (AFE) buds generated at the end of stage 2 demonstrates high expression of the anterior foregut marker, SOX2 and absence of mid/hindgut marker, CDX2. (B) Cells from branching lung organoids on day 42 (stage 4) express lung progenitor marker NKX2.1 and its surrogate cell surface marker CPM. (C) NKX2.1 and CPM expression are maintained in long-term cultures as seen by flow cytometric analysis of cells from branching lung organoids (day 67).

Panel of immunocytochemistry (ICC) images demonstrating the protein expression of key lung markers in branching lung organoids generated using STEMdiff™ Branching Lung Organoid Kit and matured using STEMdiff™ Branching Lung Organoid Maturation Kit. Panel of immunocytochemistry (ICC) images demonstrating the protein expression of key lung markers in branching lung organoids generated using STEMdiff™ Branching Lung Organoid Kit and matured using STEMdiff™ Branching Lung Organoid Maturation Kit.

Figure 3. Branching Lung Organoids Cultured in STEMdiff™ Branching Lung Organoid Kit Feature Key Protein Markers and Exhibit Branching Morphogenesis

(A) Branching lung organoids express lung progenitor marker NKX2.1 throughout their branching structures, and (B, C) demonstrate the presence of alveolar type II-like cells with Pro-surfactant protein B and C expressions. (D, E) These organoids undergo proximodistal differentiation demonstrated by the differential expression of SOX2 and SOX9. (F) MUC1 can be found luminally expressed while the (G) organoids are surrounded by VIM-expressing mesenchyme. (H, I) Branching lung organoids generated in STEMdiff™ Branching Lung Organoid Kit also express proteins associated with SARS-CoV-2 entry, ACE2 and TMPRSS2. Protein expression was visualized by immunohistochemistry and confocal microscopy of branching lung organoids on day 63.

Figure 4. Branching Lung Organoids Cultured in STEMdiff™ Branching Lung Organoid Maturation Kit For Extended Periods Express More Mature Lung Markers

(A) The branching tips of branching lung organoids derived from peripheral blood endothelial progenitor cell-derived iPS cells (iPSC-1), peripheral blood mononuclear cell-derived iPS cells (iPSC-2) or embryonic stem cells (H1 and H9) continue to grow and branch up to day 101. (B) The expression levels of more mature distal lung markers ABCA3, SFTPC, and SFTPB increase overtime. Morphology and gene expression of branching lung organoids cultured up to day 105 were assessed by RT-qPCR. RQ-values were normalized to TBP and compared to commercially available lung RNA.

Gene expression data of branching lung organoids of key lung markers like NKX2.1, MUC1, P63, FOXJ1, SFTB, SFTC, and ABCA3 and the absence of off-target markers like PAX8 and FOXG1.

Figure 5. Branching Lung Organoid Cells Are Specifically Differentiated Towards the Lung

Gene expression profile of branching lung organoids (day 42+) analysed by RT-qPCR show presence of key lung markers and absence of off-target markers. Branching lung organoids at day 42 express (A) lung-thyroid-forebrain marker NKX2.1 and does not exhibit thyroid marker PAX8 and forebrain marker FOXG1. (B) The expressions of proximal lung markers MUC1, P63, and FOXJ1 and distal lung markers SFTPC, SFTPB, and ABCA3 are upregulated in day 56 branching lung organoids, when compared to respective pluripotent stem cells (PSCs). All expression levels are normalized to the housekeeping gene TBP. iPSC-1: peripheral blood endothelial progenitor cell-derived iPS cells; iPSC-2: peripheral blood mononuclear cell-derived iPS cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Branching Lung Organoid Maturation Kit
Catalog #
100-0528
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Branching Lung Organoid Maturation Kit
Catalog #
100-0528
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Branching Lung Organoid Maturation Kit
Catalog #
100-0528
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Branching Lung Organoid Maturation Kit
Catalog #
100-0528
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

Resources and Publications

Educational Materials (22)

hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Organoids
Brochure
Organoids
STEMdiff™ Definitive Endoderm Kit
Brochure
STEMdiff™ Definitive Endoderm Kit
Pulmonary Research
Brochure
Pulmonary Research
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at the Air-Liquid Interface
Protocol
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at t...
How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
Protocol
How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Cellular Organization and Biology of the Respiratory System
Wallchart
Cellular Organization and Biology of the Respiratory System
Supporting Science Globally: A look into how scientists in Singapore are using organoids
4:46
Video
Supporting Science Globally: A look into how scientists in Singapore are using organoids
Organoid Expert Panel
52:35
Webinar
Organoid Expert Panel
hPSC-Derived Lung Models as Tools for Respiratory Research
17:17
Webinar
hPSC-Derived Lung Models as Tools for Respiratory Research
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
18:41
Webinar
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
Human Lung Development and Disease: Lessons from Organoids
26:16
Webinar
Human Lung Development and Disease: Lessons from Organoids
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
44:40
Webinar
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases, Nephrotoxicity, and Highly Relevant Cell-Based Assay
59:52
Webinar
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases,...
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
20:24
Webinar
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
Reliable In Vitro Culture Solutions for Your Alveolar Research
18:52
Webinar
Reliable In Vitro Culture Solutions for Your Alveolar Research
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
A Reproducible Protocol for the Generation of Branching Lung Organoids From Human Pluripotent Stem Cells
Scientific Poster
A Reproducible Protocol for the Generation of Branching Lung Organoids From Human Pluripotent Stem C...

更多信息

更多信息
Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。