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ReproTeSR™重编程介质（2组分）

诱导人类iPS细胞的重编程培养基
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产品号 #05926_C

诱导人类iPS细胞的重编程培养基

产品优势

  • 定义，无进料配方促进可重复高效的人类iPS细胞生成
  • 具有高质量诱导多能干细胞样形态的大集落的迅速出现，为鉴定和亚克隆提供了便利，便于建立诱导多能干细胞系
  • 在重新编程之前和iPS细胞生成之后，与STEMCELL产品无缝集成，以维持和分化
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概述

ReproTeSR™（2-Component）是一种完整的无异种重编程培养基，用于在无饲料条件下从成纤维细胞、尿源性细胞和血源性细胞（如CD34+或红细胞前体细胞）中生成人类诱导多能干细胞（iPS）。

ReproTeSR™还可作为一套集成工具的一部分，该工具针对体外扩增的外周血源性血液细胞类型进行优化，如CD34+细胞（CD34+祖细胞重编程试剂盒，目录#05925）或红细胞（红细胞祖细胞重编程试剂盒，目录#05924）。

Subtype
Specialized Media
 
Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
 
Species
Human
 
Application
Cell Culture, Reprogramming
 
Brand
TeSR
 
Area of Interest
Stem Cell Biology
 
Formulation Category
Serum-Free, Xeno-Free
 

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Data Figures

Figure 1. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean /- SEM; erythroid cells, n=4; CD34 cells, n=5.

Figure 2. ReproTeSR™ Efficiently Reprograms Fibroblasts

Dermal fibroblasts were transfected with the ReproRNA™-OKSGM vector and reprogrammed under feeder-dependent (standard KOSR-containing hES cell medium on inactivated mouse embryonic fibroblasts (iMEFs)) or feeder-independent conditions (ReproTeSR™ on Corning® Matrigel®). Fibroblasts (passage 4) were reprogrammed with average efficiencies of 0.10 ± 0.06% (hES cell medium) and 0.20 ± 0.07% (ReproTeSR™). Reprogramming efficiency of fibroblasts with ReproRNA™ and ReproTeSR™ is comparable to that reported with Sendai virus. (Schlaeger TM, et al. (2015) Nat Biotechnol 33(1): 58-63.) (n ≥ 6; Data shown are mean ± SD).

Figure 3. Feeder-Free Reprogramming with ReproRNA™-OKSGM Vector and ReproTeSR™ Generates iPS Cell Colonies with Superior Colony Morphology

Representative images of iPS cell colonies were generated using ReproRNA™‑OKSGM and cultured in (A) standard hES cell medium on irradiated mouse embryonic fibroblasts (iMEFs) or (B) ReproTeSR™ on Corning® Matrigel®. iPS cell colonies derived using ReproTeSR™ exhibit more defined borders, compact morphology, and reduced differentiation as compared to the ES cell medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free, Xeno-Free
Legal Statement: This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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