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ReproTeSR™ 重编程培养基（双组分）

用于人iPS细胞诱导的重编程培养基
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产品号 #（选择产品）

产品号 #05926_C

用于人iPS细胞诱导的重编程培养基

产品优势

  • 成分明确的无饲养层配方，有利于高效重复地生成人 iPS 细胞
  • 具有高质量 iPS 细胞样形态的大克隆的快速出现，有利于识别和亚克隆，从而轻松建立 iPS 细胞系
  • 在重新编程之前和 iPS 细胞生成之后，与 STEMCELL 产品无缝整合，便于进行维持和分化

产品组分包括

  • TeSR™-E7™/ReproTeSR™ 基础培养基，480 mL
  • ReproTeSR™ 25X 补充剂，20 mL
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ReproTeSR™（双组分）是一款完全无异源的重编程培养基，专为在无饲养层条件下，从成纤维细胞、尿液来源细胞以及血液来源细胞（例如 CD34+ 细胞或红系祖细胞）中生成人诱导性多能干细胞 (iPS) 而开发。

ReproTeSR™ 也可作为一套集成工具的一部分，该工具经过优化，可用于重编程从外周血体外扩增的血液来源细胞类型，例如 CD34+ 细胞（CD34+ 祖细胞重编程试剂盒，目录号 #05925）或红系细胞（红系祖细胞重编程试剂盒，目录号 #05924）。

亚型
专用培养基
 
细胞类型
造血干/祖细胞，多能干细胞
 
种属
人
 
应用
细胞培养，重编程
 
品牌
TeSR
 
研究领域
干细胞生物学
 
制剂类别
无血清，Xeno-Free
 

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Data Figures

Figure 1. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean /- SEM; erythroid cells, n=4; CD34 cells, n=5.

Figure 2. ReproTeSR™ Efficiently Reprograms Fibroblasts

Dermal fibroblasts were transfected with the ReproRNA™-OKSGM vector and reprogrammed under feeder-dependent (standard KOSR-containing hES cell medium on inactivated mouse embryonic fibroblasts (iMEFs)) or feeder-independent conditions (ReproTeSR™ on Corning® Matrigel®). Fibroblasts (passage 4) were reprogrammed with average efficiencies of 0.10 ± 0.06% (hES cell medium) and 0.20 ± 0.07% (ReproTeSR™). Reprogramming efficiency of fibroblasts with ReproRNA™ and ReproTeSR™ is comparable to that reported with Sendai virus. (Schlaeger TM, et al. (2015) Nat Biotechnol 33(1): 58-63.) (n ≥ 6; Data shown are mean ± SD).

Figure 3. Feeder-Free Reprogramming with ReproRNA™-OKSGM Vector and ReproTeSR™ Generates iPS Cell Colonies with Superior Colony Morphology

Representative images of iPS cell colonies were generated using ReproRNA™‑OKSGM and cultured in (A) standard hES cell medium on irradiated mouse embryonic fibroblasts (iMEFs) or (B) ReproTeSR™ on Corning® Matrigel®. iPS cell colonies derived using ReproTeSR™ exhibit more defined borders, compact morphology, and reduced differentiation as compared to the ES cell medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ReproTeSR™ Medium for Reprogramming (2-Component)
Catalog #
05926
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free, Xeno-Free
Legal Statement: This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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