MyoCult™ Differentiation Kit (Human) includes MyoCult™ Differentiation Medium and Animal Component-Free (ACF) Cell Attachment Substrate. MyoCult™ Differentiation Medium is a serum-based medium specifically formulated for the in vitro differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes. MyoCult™ Differentiation Medium is designed for use with the ACF Cell Attachment Substrate, which has been specifically tested for its ability to support optimal adherence and myotube morphology. This medium is suitable for the differentiation of myogenic progenitor cells previously expanded in serum-containing or serum-free media (e.g. using the MyoCult™-SF Expansion Kit #05980).
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
The Product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
Subtype Specialized Media
Cell Type Myogenic Stem and Progenitor Cells
Species Human
Application Cell Culture, Differentiation
Brand MyoCult
Area of Interest Disease Modeling
Data Figures
Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts
(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they
can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
