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interticult™类器官分化培养基（人）

细胞培养基进一步分化人肠道类器官在三维，或作为单层/气液界面培养
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产品号 #（选择产品）

产品号 #100-0214_C

细胞培养基进一步分化人肠道类器官在三维，或作为单层/气液界面培养

产品优势

  • 使肠道类器官的培养具有生理比例的分化和干细胞群体
  • 通过将肠道类器官转化为单层和ALI培养物，可以很容易地进入根尖表面
  • 在传代和复制中产生一致的结果

产品组分包括

  • interticult™ODM人基础培养基，50 mL
  • 类器官补充剂，50毫升
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. IntestiCult™ Organoid Growth Medium (Human)
    IntestiCult™ Organoid Growth Medium (Human)

    Cell culture medium for establishment and maintenance of human intestinal organoids

  2. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  3. Costar® 6.5 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts
    Costar® 6.5 mm Transwell®, 0.4 µm Pore Pol...

    Polystyrene plate with lid and polyester membrane inserts for cell culture that feed basolaterally

  4. Y-27632 (Dihydrochloride)
    Y-27632 (Dihydrochloride)

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

What Our Scientist Says

Organoids have truly expanded the limits of what's possible for in vitro studies of the intestinal epithelium. By providing optimized culture media and robust, approachable protocols, we are making these technologies more accessible to researchers.

Ryan ConderAssociate Director, Gastrointestinal Biology
Ryan Conder, Associate Director
总览
实验数据
产品说明书及文档
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概述

inteticult™类器官分化培养基（人）是一种完整的培养基，支持在三维（3D）或二维(单层或气液界面（ALI）培养中进一步分化肠道类器官。起始培养物可以是来源于人肠隐窝的类肠道器官，也可以是与interticult™类器官生长培养基(人类;目录# 06010)。

使用肠系膜™类器官分化培养基（人）生成的肠道培养物含有生理相关比例的分化细胞和干细胞群，概括了隐窝绒毛轴的多样性。与传统细胞系相比，肠单层细胞系表现出更大的屏障完整性，表达更高水平的关键分化标志物，并且具有更能代表体内肠道的形态学。

肠道类器官培养的应用包括研究肠上皮的发育和功能，模拟肠道疾病，化合物筛选和再生治疗方法。肠道单层培养物和ALI培养物特别适合于渗透性测定和传染病研究，因为它们易于接触到根尖表面。该试剂盒需要inteticult™类器官生长培养基(人；目录#06010)，用于肠道类器官分化前的起始和扩张。

学习如何培养人类肠道类器官按需肠道疗程或浏览我们的常见问题（FAQs）关于使用inteticult™的类器官工作流程。此外，下载我们详细的电子书经过验证的肠道类器官培养方案：开始使用inteticult™肠道类器官方案的精选集。

如果您打算将本产品用于商业用途，请与HUB Organoids B.V.联系www.huborganoids.nl申请商业用途许可证或澄清与HUB Organoids B.V.许可有关的信息。

Subtype
Specialized Media
 
Cell Type
Intestinal Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion, Maintenance, Organoid Culture
 
Brand
IntestiCult
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology
 

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Data Figures

Differentiated Human Intestinal Organoids Display a Budded Morphology

Figure 1. Differentiated Human Intestinal Organoids Display a Budded Morphology

(A) Organoids grown in IntestiCult™ OGM are primarily cystic. (B) When switched to IntestiCult™ ODM, organoids develop a thickened epithelium with a pronounced, budded morphology indicative of a more differentiated state. Organoids were imaged on day 5 of expansion or differentiation respectively.

Intestinal Organoids Contain a Higher Proportion of Mature Cell Types Following Differentiation in IntestiCult™ ODM

Figure 2. Intestinal Organoids Contain a Higher Proportion of Mature Cell Types Following Differentiation in IntestiCult™ ODM

(A, B) Organoids grown in IntestiCult™ OGM are enriched for Ki-67+ proliferative cells (A), while containing few differentiated cell types such as goblet cells (MUC2, A), enterocytes (KRT20, B), and enteroendocrine cells (CHGA, B). (C, D) When switched to IntestiCult™ ODM, organoids contain a small number of Ki-67+ proliferative cells (C, arrows), with more physiological proportions of goblet cells (MUC2, C), enterocytes (KRT20, D), and chromogranin A- (CHGA-)positive enteroendocrine cells (D, arrow).

Differentiation of Intestinal Epithelium at the Air-Liquid Interface (ALI) Using IntestiCult™ ODM

Figure 3. Differentiation of Intestinal Epithelium at the Air-Liquid Interface (ALI) Using IntestiCult™ ODM

(A – E) Growing organoid-derived monolayers as ALI cultures drives further differentiation of intestinal epithelial cultures as seen by changes in gene expression measured by RT-qPCR. Relative quantification (RQ) for each marker is shown relative to actB and TBP housekeeping genes and normalized with respect to undifferentiated organoids grown in IntestiCult OGM (Human). Progenitor markers (A) Lgr5 and (B) Axin2 are significantly reduced in both submerged monolayers and ALI cultures, while markers of enterocytes (KRT20, C), goblet cells (MUC2, D), and enteroendocrine cells (CHGA, E) are significantly increased. Further reduction in Axin2 is seen in ALI monolayers with an increase in expression of KRT20, MUC2, and CHGA. (F, G) Comparing cross-sections of organoid monolayers grown in IntestiCult™ ODM as (F) submerged culture or (G) at the ALI shows further differentiation of the intestinal epithelium with an increased proportion of goblet cells and extracellular mucus (MUC2, green).

Differentiated Organoid-Derived Monolayers and ALI Cultures Display More Physiological Trans-Epithelial Electrical Resistance (TEER) than Caco-2 Cells

Figure 4. Differentiated Organoid-Derived Monolayers and ALI Cultures Display More Physiological Trans-Epithelial Electrical Resistance (TEER) than Caco-2 Cells

Differentiated organoid-derived monolayers grown as a submerged monolayer (IntestiCult™ ODM Monolayer), or at the ALI (IntestiCult™ ODM ALI), show higher TEER values as compared to Caco-2 cultures.Organoid-derived monolayers grown at the ALI show a loosening of tight junctions due to further differentiation of the brush border, and thus lower TEER values are observed. * p < 0.0001.

Differentiated Intestinal Organoids Provide a Suitable Model for Studying CFTR Response In Vitro

Figure 5. Differentiated Intestinal Organoids Provide a Suitable Model for Studying CFTR Response In Vitro

(A) Organoids differentiated further in IntestiCult™ ODM show a comparable degree of swelling when treated with forskolin as compared to organoids grown in IntestiCult™ OGM, demonstrating suitability for use in forskolin-induced swelling assays. (B – E) Ussing chamber analysis of submerged (B) organoid-derived monolayers and (C) Caco-2 cultures demonstrate increased sensitivity of organoid-derived monolayers to CFTR activation and inhibition by IBMX/Forskolin and CFTR Inhibitor-172 respectively. (D, E) Analysis of CFTR modulation by IBMX/Forskolin and CFTR Inhibitor-172 show significantly greater (D) activation and (E) inhibition of CFTR activity in organoid-derived monolayers as compared to Caco-2 cultures (p < 0.001 for both).

Data and images of the MIMETAS OrganoReady Colon Organoid Plate

Figure 6. The MIMETAS OrganoReady® Colon Organoid Platform Uses IntestiCult™ to Create an Advanced Physiologically Relevant Model for Gastrointestinal Toxicity Testing and Barrier Integrity

(A) The OrganoReady® plate highlighting the microfluidic compartments.

(B) Schematic of the OrganoReady® microfluidic compartments where columns 1, 2, and 3 house the medium, a collagen-1 matrix, and the colon organoid tubule, respectively.

(C) Immunofluorescence staining of the colon organoid tubule confirms an adult tissue phenotype with the presence of goblet cells (Muc2), enterocytes (Occludin), and stem cells (Sox9). The 3D-lumenized structure provides apical (Ezrin) and basolateral (Integrin-β4) access to the polarized epithelium. Additionally, the organoid tubules show polarized and modulatable activity of expression of P-glycoprotein (Pgp).

(D) The OrganoReady® Colon Organoid platform supports toxicity testing, as demonstrated by dose-dependent measurements of TEER, LDH, and ATP following exposure to Afatinib (n = 4, N = 2). After 72 hrs of exposure, a dose dependent decrease in TEER, cytotoxicity, and cell viability was observed. For more information, please visit mimetas.com/en/organoready-organoid/.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
IntestiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0214
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
IntestiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0214
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
IntestiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0214
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (52)

Organoids
Brochure
Organoids
Forskolin-Induced Swelling of Human Intestinal Organoids Grown in IntestiCult™
Technical Bulletin
Forskolin-Induced Swelling of Human Intestinal Organoids Grown in IntestiCult™
Culturing Intestinal Cancer-Derived Organoids
Protocol
Culturing Intestinal Cancer-Derived Organoids
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
How to Isolate Human Colonic Crypts from Intestinal Biopsies
Protocol
How to Isolate Human Colonic Crypts from Intestinal Biopsies
How to Cryopreserve Intestinal Organoids
Protocol
How to Cryopreserve Intestinal Organoids
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at the Air-Liquid Interface
Protocol
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at t...
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
Protocol
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
How to Thaw Intestinal Organoids
Protocol
How to Thaw Intestinal Organoids
How to Generate Monolayers from hPSC-Derived Organoids Using IntestiCult™
Protocol
How to Generate Monolayers from hPSC-Derived Organoids Using IntestiCult™
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
SnapShot: GI Tract Development
Wallchart
SnapShot: GI Tract Development
SnapShot: The Intestinal Crypt
Wallchart
SnapShot: The Intestinal Crypt
SnapShot: Growing Organoids from Stem Cells
Wallchart
SnapShot: Growing Organoids from Stem Cells
Correlating TEER Values with Air-Liquid Interface Culture Morphology
3:02
Video
Correlating TEER Values with Air-Liquid Interface Culture Morphology
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
3:19
Video
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
How to Count Intestinal Crypts for Mouse Organoid Cultures
1:09
Video
How to Count Intestinal Crypts for Mouse Organoid Cultures
Collaborating to Accelerate COVID-19 Research
3:36
Video
Collaborating to Accelerate COVID-19 Research
How to Perform a TEER Measurement to Evaluate Epithelial Barrier Integrity in ALI Cultures
4:25
Video
How to Perform a TEER Measurement to Evaluate Epithelial Barrier Integrity in ALI Cultures
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Development
11:52
Webinar
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Developm...
Nature Research Round Table: Organoids as Models for Human Disease
15:10
Webinar
Nature Research Round Table: Organoids as Models for Human Disease
Molecular Tricks of Neurotropic Intestinal Viruses
25:54
Webinar
Molecular Tricks of Neurotropic Intestinal Viruses
Organoid Mini-Symposium: Advanced Organoid Culture Systems and Their Applications in Infectious Diseases
32:21
Webinar
Organoid Mini-Symposium: Advanced Organoid Culture Systems and Their Applications in Infectious Dise...
Organoid-Based Assays for Disease Modeling
24:55
Webinar
Organoid-Based Assays for Disease Modeling
Spotlight on Organoids: Expert Panel
Webinar
Spotlight on Organoids: Expert Panel
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
Applications and Analysis of Organoids
47:16
Webinar
Applications and Analysis of Organoids
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
4:47
Webinar
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
Applications of Human Intestinal Organoid Cultures in Drug Discovery
56:20
Webinar
Applications of Human Intestinal Organoid Cultures in Drug Discovery
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
10:17
Webinar
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
Optimizing Organoid Models to Better Predict Clinical Outcomes
55:09
Webinar
Optimizing Organoid Models to Better Predict Clinical Outcomes
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
24:56
Webinar
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Organoid Mini-Symposium: Human Intestinal Organoids to Study Rare Gut Endocrine Cells
Webinar
Organoid Mini-Symposium: Human Intestinal Organoids to Study Rare Gut Endocrine Cells
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
47:49
Webinar
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
Organoid Expert Panel
52:35
Webinar
Organoid Expert Panel
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
15:15
Webinar
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
56:31
Webinar
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
Nature Research Round Table: Progress and Challenges in Organoid Models of Human Brain Development
9:36
Webinar
Nature Research Round Table: Progress and Challenges in Organoid Models of Human Brain Development
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
1:03:40
Webinar
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Challenges in Translating iPSC Technology
41:47
Webinar
Challenges in Translating iPSC Technology
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Bioelectronic Tools to Study the Gut-Brain Axis
40:52
Webinar
Bioelectronic Tools to Study the Gut-Brain Axis
Organoid Mini-Symposium: Utilization of Next Generation CRISPR Tools to Model Health and Disease in Adult Stem Cell-Derived Organoids
Webinar
Organoid Mini-Symposium: Utilization of Next Generation CRISPR Tools to Model Health and Disease in ...
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
17:32
Webinar
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
Patient-Derived Organoids for Drug Screening and Development
50:25
Webinar
Patient-Derived Organoids for Drug Screening and Development
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Scientific Poster
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-g...
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
E-Book
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
Human Intestinal Organoid Course
On-Demand Training
Human Intestinal Organoid Course

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Species Human
Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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