Trypsin-EDTA (0.05%)

Enzymatic cell dissociation reagent

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  • Generate single-cell suspenstions of human ES and iPS cells for downstream assays with a gentle, low concentration Trypsin-EDTA solution.

  • Generate single-cell suspensions of human embryonic stem cells (ES) or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays with Trypsin-EDTA (0.05%). EDTA weakens cell-cell adhesion, which improves trypsin's access to hydrolysis-targeted peptide bonds, and enhances detachment of adherent cells. This is a gentler version than the standard concentration (0.25%; Catalog #07901), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.
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    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Catalog #
    07910
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Catalog #
    07910
    Lot #
    All
    Language
    English

    Resources and Publications

    Publications (1)

    Intermediate DNA methylation is a conserved signature of genome regulation Elliott G et al. Nature Communications 2015 DEC

    Abstract

    The role of intermediate methylation states in DNA is unclear. Here, to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity, we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers, exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation, are predominantly allele-independent and are conserved across individuals and between mouse and human, suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons, highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.
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