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AggreWell™HT

高通量96孔微孔培养板，易于复制胚状体和球体的生产
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产品号 #200-0563_C

高通量96孔微孔培养板，易于复制胚状体和球体的生产

产品优势

  • 900 μm微井简化了高通量工作流程，每口井可形成32个聚集体，非常适合扩大规模
  • 确保重复性与一致和一致大小的细胞聚集体
  • 增强可视化与半月板减少功能优越的亮场图像
  • 容易形成聚集体，每微孔只有50个细胞
  • 无缝过渡与跨AggreWell™格式的兼容性

产品组分包括

  • AggreWell™HT 96孔板
    • 1台（目录号200-0563）
    • 5个（目录号200-0570）
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Anti-Adherence Rinsing Solution
    Anti-Adherence Rinsing Solution

    Rinsing solution for cultureware to prevent cell adhesion

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实验数据
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概述

使用AggreWell™HT简化3D培养工作流程，这是一种高通量96孔微孔板，旨在优化均匀细胞聚集体的形成。无论生成的是椭球体还是胚状体（EBs）， AggreWell™HT每口井最多可生成32个聚集体——比传统的超低附着板要多——从而获得更高的统计能力、更小的可变性和更可靠的结果。

AggreWell™HT非常适合稀有细胞类型或有限样品的实验，每微孔仅需50个细胞，确保您可以最大限度地利用样品。该板还与机器人液体处理系统兼容，其减少半月板的功能提高了明场成像的质量和易用性，进一步提高了您的工作效率。注：防黏附漂洗液(目录# 07010)是最佳的EB和球体形成所必需的。

AggreWell™HT与两国开发的应用程序兼容AggreWell™400和AggreWell™800格式，允许有效地扩展工作流。了解更多关于如何使用AggreWell™或联系我们了解更多信息。

Subtype
Dishes and Plates
 
Application
Cell Culture, Characterization, Differentiation, Organoid Culture, Spheroid Culture, Toxicity Assay
 
Brand
AggreWell
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
 

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Data Figures

AggreWell™HT features a meniscus-reducing feature and allows for the formation of aggregates from various initial seeding densities.

Figure 1. AggreWell™HT Promotes Cell Aggregation Across 32 Individual Microwells

(A) AggreWell™HT single-well cross section displaying the microwell array, featuring 32 microwells, and the meniscus-reducing step feature. Visualization of human embryonic stem cell (hESC) aggregates formed in AggreWell™HT after 72 hours from an initial seeding density of (B) 1,000 cells per microwell and (C) 10,000 cells per microwell.

AggreWell™HT promotes neural induction of embryonic stem cells to the same extent as AggreWell™800.

Figure 2. AggreWell™HT Promotes Neural Induction in Embryoid Bodies Comparable to AggreWell™800

Human embryonic stem (ES) cell (H9) aggregates cultured in (A) AggreWell™HT and (B) AggreWell™800 microwell plates 24 hours post-seeding; scale bar = 500 μm. Morphology of Day 6 neural progenitor cell rosettes differentiated using an embryoid body (EB)-based protocol with Neural Induction Medium (Catalog #05835) in (C) AggreWell™HT and (D) AggreWell™800 microwell plates; scale bar = 500 μm. (E) Neural marker expression of PAX6 and SOX1 is equivalent in both AggreWell™ plates at Day 18 following neural induction of H9 ES cells in both AggreWell™HT and AggreWell™800 plates, as determined by flow cytometry (n = 4). Error bars = SD.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
AggreWell™HT 96-well plate, 1 pack
Catalog #
200-0563
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
AggreWell™HT 96-well plate, 5 pack
Catalog #
200-0570
Lot #
All
Language
English
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