AggreWell™800

简单可重复性地制备拟胚体和球体的微孔培养板

产品号 #(选择产品)

产品号 #34811_C

简单可重复性地制备拟胚体和球体的微孔培养板

产品组分包括

  • AggreWell™800 24孔板
    • 1块(产品号#34811)
    • 5块(产品号#34815)
  • AggreWell™800 6孔板
    • 1块(产品号#34821)
    • 5块(产品号#34825)
  • AggreWell™800 24孔板套装(产品号#34850)
    • 2 x 24孔板
    • 1瓶防粘附冲洗液(产品号#07010)
  • AggreWell™800 6孔板套装(产品号#34860)
    • 2 x 6孔板
    • 1瓶防粘附冲洗液(产品号#07010)
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品,请参阅《实验方案与技术文档》
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总览

AggreWell™细胞培养板提供了一种简单、标准化的方法来生成细胞聚集体,包括拟胚体(EBs)和球体。使用AggreWell™板生成的EBs和球体在大小和形状上是均一的,并且在实验内和实验间均表现出良好的均一性。新一代改进版 AggreWell™ 板兼容多种细胞类型,包括胚胎干细胞、诱导多能干细胞、肿瘤细胞等。其增强的光学特性确保成像清晰无杂质。注意:为了获得最佳的拟胚体和球体形成效果,需配合使用 AggreWell™ 抗粘附冲洗液。.  

如果您使用AggreWell™400板,请参见在这里.

亚型
培养皿与培养板
 
种属
人,小鼠,非人灵长类,其它细胞系,大鼠
 
应用
分化,球状体培养,细胞毒性检测
 
品牌
AggreWell
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
34825
Lot #
All
Language
English
Catalog #
34811
Lot #
All
Language
English
Catalog #
34860
Lot #
All
Language
English
Catalog #
34821
Lot #
All
Language
English
Catalog #
34815
Lot #
All
Language
English
Catalog #
34850
Lot #
All
Language
English

Resources and Publications

Educational Materials (20)

Publications (8)

Modelling Lyssavirus Infections in Human Stem Cell-Derived Neural Cultures. V. Sundaramoorthy et al. Viruses 2020 mar

Abstract

Rabies is a zoonotic neurological infection caused by lyssavirus that continues to result in devastating loss of human life. Many aspects of rabies pathogenesis in human neurons are not well understood. Lack of appropriate ex-vivo models for studying rabies infection in human neurons has contributed to this knowledge gap. In this study, we utilize advances in stem cell technology to characterize rabies infection in human stem cell-derived neurons. We show key cellular features of rabies infection in our human neural cultures, including upregulation of inflammatory chemokines, lack of neuronal apoptosis, and axonal transmission of viruses in neuronal networks. In addition, we highlight specific differences in cellular pathogenesis between laboratory-adapted and field strain lyssavirus. This study therefore defines the first stem cell-derived ex-vivo model system to study rabies pathogenesis in human neurons. This new model system demonstrates the potential for enabling an increased understanding of molecular mechanisms in human rabies, which could lead to improved control methods.
Maturation of Human Pluripotent Stem Cell-Derived Cerebellar Neurons in the Absence of Co-culture. T. P. Silva et al. Frontiers in bioengineering and biotechnology 2020

Abstract

The cerebellum plays a critical role in all vertebrates, and many neurological disorders are associated with cerebellum dysfunction. A major limitation in cerebellar research has been the lack of adequate disease models. As an alternative to animal models, cerebellar neurons differentiated from pluripotent stem cells have been used. However, previous studies only produced limited amounts of Purkinje cells. Moreover, in vitro generation of Purkinje cells required co-culture systems, which may introduce unknown components to the system. Here we describe a novel differentiation strategy that uses defined medium to generate Purkinje cells, granule cells, interneurons, and deep cerebellar nuclei projection neurons, that self-formed and differentiated into electrically active cells. Using a defined basal medium optimized for neuronal cell culture, we successfully promoted the differentiation of cerebellar precursors without the need for co-culturing. We anticipate that our findings may help developing better models for the study of cerebellar dysfunctions, while providing an advance toward the development of autologous replacement strategies for treating cerebellar degenerative diseases.
A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues. M. Robinson et al. Biosensors 2019 apr

Abstract

We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Neurons require soft substrates to differentiate and mature, however measuring the elastic moduli of soft substrates remains difficult to accurately measure using standard protocols such as atomic force microscopy or shear rheology. Here we validate a direct method for acquiring elastic modulus of fibrin using a modified Hertz model for thin films. In this method, spherical indenters are positioned on top of the fibrin samples, generating an indentation depth that is then correlated with elastic modulus. Neurons function by transmitting electrical signals to one another and being able to assess the development of electrical signaling serves is an important verification step when engineering neural tissues. We then validated a protocol wherein the electrical activity of motor neural cultures is measured directly by a voltage sensitive dye and a microplate reader without causing damage to the cells. These protocols provide a non-destructive method for characterizing the mechanical and electrical properties of living spinal cord tissues using novel biosensing methods.

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更多信息
Species Human, Mouse, Non-Human Primate, Other, Rat
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