产品号 #78072_C
Thrombopoietin
概述
Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes and has an important role in regulating the level of circulating platelets in vivo. TPO also promotes the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitor cells. It is commonly used with other cytokines such as stem cell factor (SCF) and Flt3/Flk-2 Ligand to promote expansion of primitive hematopoietic cells in culture (Hitchcock & Kaushansky). The TPO receptor, c-Mpl, is expressed at all stages of megakaryopoiesis, from hematopoietic stem and progenitor cells to mature platelets.
Subtype
Cytokines
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Mouse
Area of Interest
Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Mouse Recombinant TPO was tested by its ability to promote the proliferation of MO7e cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.92 ng/mL. (B) 5 μg of Mouse Recombinant TPO was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant TPO has a predicted molecular mass of 35 kDa but as a result of glycosylation has an apparent molecular mass of approximately 75 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mouse Recombinant TPO Catalog #
78072.2, 78072, 78072.1 Lot #
1000112118 or higher Language
English Document Type
Safety Data Sheet Product Name
Mouse Recombinant TPO Catalog #
78072.2, 78072, 78072.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Resources and Publications
Educational Materials (1)
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