The Mouse Immunoglobulin M (IgM) ELISA Antibody Pair Kit is intended for those who want the flexibility of setting up their own ELISA assay. This kit includes capture and detection antibodies and an IgM standard. It is designed for the quantitative detection and measurement of mouse IgM in biological fluids such as serum, plasma, and cell culture supernatants. IgM is predominantly found in lymph fluid and in blood where it acts as a neutralizing agent in the early stages of infection. Secreted IgM exists in a pentameric form whereas monomeric IgM is found on the surface of B cells. IgM constitutes approximately 10% of all serum immunoglobulins.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates (Catalog #38019). ELISA plates and pNPP Substrate (Catalog #01917) are required for use with the Mouse IgM ELISA Antibody Pair Kit and are available for purchase separately.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA plates coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate p-nitrophenyl phosphate (pNPP) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
NOTE: This kit includes sufficient reagents for 6 x 96-well ELISA plates (Catalog #38019). ELISA plates and pNPP Substrate (Catalog #01917) are required for use with the Mouse IgM ELISA Antibody Pair Kit and are available for purchase separately.
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Mouse IgM ELISA Antibody Pair Kit
Catalog #
01999
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (2)
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