产品号 #01997_C
用于小鼠免疫球蛋白E的检测和测定
New! For your convenience, all of our antibody pair kits now include a vial of Standard Reconstitution Buffer B. This change does not affect product performance or quality.
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
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pNPP ELISA SubstrateSubstrate for ELISA antibody pair kits
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
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Corning® 96-Well High-Binding Flat-Bottom Mi...Clear polystyrene flat-bottom, high-binding microplate without lid
概述
小鼠免疫球蛋白E (IgE) ELISA抗体对试剂盒是为那些谁想要建立自己的ELISA测定的灵活性。该试剂盒包括捕获和检测抗体和IgE标准。它是专为定量检测和测量小鼠IgE的生物液体，如血清，血浆和细胞培养上清。IgE主要存在于肺、皮肤和粘膜中，是血清中最不常见的免疫球蛋白。IgE通过与Fcε受体I （Fcε ri）相互作用，刺激嗜酸性粒细胞、肥大细胞和嗜碱性粒细胞的脱颗粒，在过敏反应中发挥重要作用。
该检测基于夹心ELISA方法，将样品添加到涂有免疫球蛋白特异性捕获抗体的ELISA板上。捕获的免疫球蛋白通过添加生物素化检测抗体进行检测，然后添加链霉亲和素碱性磷酸酶（SA-ALP），该酶与生物素化抗体结合。添加显色酶底物对硝基苯基磷酸（pNPP）可得到着色产物，其强度与样品中免疫球蛋白的浓度成正比。免疫球蛋白的浓度是通过与平行分析的免疫球蛋白标准品的连续稀释度进行比较来确定的。
注意：该试剂盒包括足够的试剂6 × 96孔ELISA板（目录#38019）。ELISA板和pNPP底物（目录#01917）需要与小鼠IgE ELISA抗体对试剂盒一起使用，并可单独购买。
Subtype
Antibody Pair Kits
Cell Type
B Cells, Hybridomas
Species
Mouse
Area of Interest
Hybridoma Generation, Immunology
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
更多信息
| Species | Mouse |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
