MesenCult™-hPL Medium has been optimized for the culture and expansion of human mesenchymal stromal cells (MSCs; also known as mesencyhymal stem cells) as well as for the detection and enumeration of colony-forming unit fibroblasts (CFU-F). MesenCult™-hPL 10X Supplement is an alternative growth supplement to fetal bovine serum (FBS) that contains purified human platelet lysate (hPL). Complete MesenCult™-hPL Medium (Basal Medium + 10X Supplement) does not require addition of growth factors, lipids, or attachment substrate. It is more defined and provides better performance than FBS-containing media, and is also a more affordable option than defined, serum-free media.
Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Subtype Specialized Media
Cell Type Mesenchymal Stem and Progenitor Cells
Species Human
Application Cell Culture, Colony Assay, Expansion
Brand MesenCult
Area of Interest Stem Cell Biology
Data Figures
Figure 1. Bone marrow (BM)-derived MSCs Cultured in MesenCult™-hPL Medium Expand Faster than MSCs Cultured in Serum (FBS)-containing medium
Figure 2. Human BM-derived MSCs Cultured in MesenCult™-hPL Medium Display Multi-Lineage Differentiation Potential
(A) Human BM-derived MSCs cultured in MesenCult™-HPL Medium differentiate into (B) adipocytes (Oil Red O staining); and (C) chondrocytes (Alcian Blue staining); and (D) osteoblasts (alkaline phosphatase and von Kossa staining).
Figure 3. Flow Cytometric Analysis of Cultured-Expanded MSCs in MesenCult™-hPL Medium
BM-derived MSCs were cultured and expanded in MesenCult™-hPL Medium. MSCs were stained for the mesenchymal surface markers CD73, CD90 and CD105, and the hematopoietic marker, CD45. MSCs express high levels of CD73, CD90 and CD105 while lacking expression of CD45.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Improved therapeutic consistency and efficacy of mesenchymal stem cells expanded with chemically defined medium for systemic lupus erythematosus. J. Xu et al. Cellular {\&} molecular immunology 2020 oct
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they
can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
