MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.
MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.
NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
The Product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
Subtype Specialized Media
Cell Type Mesenchymal Stromal Cells
Species Human
Application Cell Culture, Cell Isolation
Brand MesenCult
Area of Interest Cell Line Development, Stem Cell Biology
Formulation Category Animal Component-Free
Data Figures
Figure 1. Workflow for Expanding UC-MSCs Using the MesenCult™-ACF Plus Umbilical Cord Culture Kit
Protocol for deriving and expanding umbilical cord-derived mesenchymal stromal cells (UC-MSCs) with the MesenCult™-ACF Plus Umbilical Cord Culture Kit. UC-MSCs are derived in 14 days using MesenCult™-ACF Plus Medium supplemented with the MesenCult™-ACF Plus Umbilical Cord Derivation 500X Supplement. After Day 14, cells can be maintained in complete MesenCult™-ACF Plus Medium. Representative images at Day 6, 14, and 21 are included for reference. Click here for the full protocol.
Figure 2. High UC-MSC Yield Is Observed When Isolated with MesenCult™-ACF Plus Umbilical Cord Culture Kit
Compared to MesenCult™-ACF Plus (MACFP) medium, the isolation yield, per gram umbilical cord tissue, of UC-MSCs in MesenCult™-ACF Plus Umbilical Cord (MACFP-UC) Culture Kit is 65% higher. The addition of 2.5% human AB serum during isolation further increases the yield to over 4 times more than with MesenCult™-ACF Plus alone.
Figure 3. Consistent Growth and Morphology for over 20 Passages of UC-MSCs Cultured with the MesenCult™-ACF Umbilical Cord Culture Kit
(A) MesenCult™-ACF Plus Umbilical Cord Culture Kit supports the long-term expansion of UC-MSCs. UC-MSCs isolated and expanded according to the MesenCult™-ACF Plus Umbilical Cord Culture Kit protocol can be cultured for up to 23 passages. (B) The morphology of UC-MSCs from two donors remains MSC-like following 21 passages in MesenCult™-ACF Plus Medium.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
