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MesenCult™-ACF Plus脐带MSC培养试剂盒

从脐带组织中衍生和扩增人间充质间质细胞的无动物成分培养基
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产品号 #（选择产品）

产品号 #100-0234_C

从脐带组织中衍生和扩增人间充质间质细胞的无动物成分培养基

产品优势

  • 支持脐带组织中MSCs的高效衍生；
  • 脐带衍生的MSCs始终表达MSC地特异性标记物，并保持优越的扩增和分化能力；
  • 完整无动物成分（ACF）产品线，与MesenCult™-ACF产品兼容

产品组分包括

  • MesenCult™-ACF Plus 培养基, 500 mL；
  • MesenCult™脐带MSC衍生500X补充剂，0.5 mL；
  • MesenCult™-ACF Plus 500X补充剂，0.5 mL；
  • 无动物成分细胞贴附基质 ，3 × 1ml
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

MesenCult™-ACF Plus脐带MSC培养试剂盒是包括标准化的无动物成分的无血清培养基，用于脐带组织来源的人间充质基质细胞(也称为间充质干细胞)的衍生和扩增。该试剂盒需与完整的MesenCult™-ACF Plus 培养基一起使用，用于脐带组织来源 MSCs 的长期扩增。MesenCult™-ACF Plus脐带MSC培养试剂盒必须与无动物成分细胞贴附基质和无动物成分细胞解离试剂盒（产品号 #05426）一起使用，以提供完整的无动物成分（ACF）培养系统。使用MesenCult™-ACF Plus脐带MSC培养试剂盒和MesenCult™-ACF Plus培养基培养细胞时，为了获得最佳的细胞贴附，我们预先筛选和测试了无动物成分细胞贴附基质和无动物成分细胞解离试剂盒的组分。为了获得最佳的无动物成分细胞冻存体系，建议使用MesenCult™-ACF冻存液（产品号 #05490）冻存之前培养在MesenCult™培养基中的细胞。

注：需要L-谷氨酰胺（产品号 #07100）用于制备MesenCult™-ACF Plus脐带MSC培养基和MesenCult™-ACF Plus完全培养基；参见产品说明试剂和材料的准备部分。

CollPlant是细胞贴附基质中重组人胶原蛋白(rhCollagen)组分的生产商。

本产品仅供研究使用。有关本产品的任何临床或商业应用，请联系STEMCELL。

亚型
专用培养基
 
细胞类型
间充质基质细胞
 
种属
人
 
应用
细胞培养，细胞分选
 
品牌
MesenCult
 
研究领域
细胞系制备，干细胞生物学
 
制剂类别
Animal Component-Free
 

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Data Figures

Figure 1. Workflow for Expanding UC-MSCs Using the MesenCult™-ACF Plus Umbilical Cord Culture Kit

Protocol for deriving and expanding umbilical cord-derived mesenchymal stromal cells (UC-MSCs) with the MesenCult™-ACF Plus Umbilical Cord Culture Kit. UC-MSCs are derived in 14 days using MesenCult™-ACF Plus Medium supplemented with the MesenCult™-ACF Plus Umbilical Cord Derivation 500X Supplement. After Day 14, cells can be maintained in complete MesenCult™-ACF Plus Medium. Representative images at Day 6, 14, and 21 are included for reference. Click here for the full protocol.

Figure 2. High UC-MSC Yield Is Observed When Isolated with MesenCult™-ACF Plus Umbilical Cord Culture Kit

Compared to MesenCult™-ACF Plus (MACFP) medium, the isolation yield, per gram umbilical cord tissue, of UC-MSCs in MesenCult™-ACF Plus Umbilical Cord (MACFP-UC) Culture Kit is 65% higher. The addition of 2.5% human AB serum during isolation further increases the yield to over 4 times more than with MesenCult™-ACF Plus alone.

Figure 3. Consistent Growth and Morphology for over 20 Passages of UC-MSCs Cultured with the MesenCult™-ACF Umbilical Cord Culture Kit

(A) MesenCult™-ACF Plus Umbilical Cord Culture Kit supports the long-term expansion of UC-MSCs. UC-MSCs isolated and expanded according to the MesenCult™-ACF Plus Umbilical Cord Culture Kit protocol can be cultured for up to 23 passages. (B) The morphology of UC-MSCs from two donors remains MSC-like following 21 passages in MesenCult™-ACF Plus Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Animal Component-Free
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