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MegaCult™-C Complete Kit with Cytokines

Complete kit for human CFU-Mk assays
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产品号 #04971_C

Complete kit for human CFU-Mk assays

产品组分包括

  • MegaCult™-C Complete Kit with Cytokines (Catalog #04971)
    • Collagen Solution, 35 mL (Catalog #04902)
    • MegaCult™-C Medium with Cytokines, 24 x 2 mL (Catalog #04901)
    • Double Chamber Slide Kit (Catalog #04963)
    • MegaCult™-C Staining Kit for CFU-Mk (Catalog #04962)
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MegaCult™-C Complete Kit with Cytokines
概述
产品说明书及文档
应用领域
相关材料与文献

概述

MegaCult™-C Complete Kit with Cytokines includes all reagents required for the optimal growth of megakaryocyte progenitors (CFU-Mk) within double chamber slides and for the detection of CFU-Mk colonies by immunocytochemical staining.

MegaCult™-C medium is optimized for the growth of CFU-Mk in human bone marrow, mobilized peripheral blood and cord blood samples. It is suitable for use with CD34+-enriched cells, mononuclear cells and cells isolated by other purification methods.

Contains
• Collagen Solution, 35 mL (Catalog #04902) <br> • MegaCult™-C Medium with Cytokines, 24 x 2 mL (Catalog #04901) <br> • Double Chamber Slide Kit, 1 Kit (Catalog #04963) <br> • MegaCult™-C Staining Kit for CFU-Mk, 1 Kit (Catalog #04962)
 
Subtype
Semi-Solid Media, Specialized Media
 
Cell Type
Hematopoietic Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture, Colony Assay, Functional Assay
 
Brand
MegaCult
 
Area of Interest
Stem Cell Biology
 
Formulation Category
Serum-Free
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
MegaCult™-C Complete Kit with Cytokines
Catalog #
04971
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Publications (31)

Identification of unipotent megakaryocyte progenitors in human hematopoiesis. Miyawaki K et al. Blood 2017 MAR

Abstract

The developmental pathway for human megakaryocytes remains unclear and the definition of pure unipotent megakaryocyte progenitor is still controversial. Using single-cell transcriptome analysis, we have identified a cluster of cells within immature hematopoietic stem and progenitor cell populations that specifically express genes related to the megakaryocyte lineage. We used CD41 as a positive marker to identify these cells within the CD34(+)CD38(+)IL-3Rα(dim)CD45RA(-) common myeloid progenitor (CMP) population. These cells lacked erythroid and granulocyte/macrophage potential, but exhibited robust differentiation into the megakaryocyte lineage at a high frequency, both in vivo and in vitro The efficiency and expansion potential of these cells exceeded those of conventional bipotent megakaryocyte/erythrocyte progenitors. Accordingly, the CD41(+) CMP was defined as a unipotent megakaryocyte progenitor (MegP) that is likely to represent the major pathway for human megakaryopoiesis, independent of canonical megakaryocyte-erythroid lineage bifurcation. In the bone marrow of patients with essential thrombocythemia, the MegP population was significantly expanded in the context of a high burden of Janus kinase 2 mutations. Thus, the prospectively isolatable and functionally homogeneous human MegP will be useful for the elucidation of the mechanisms underlying normal and malignant human hematopoiesis.
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Serum response factor is an essential transcription factor in megakaryocytic maturation. Halene S et al. Blood 2010 SEP

Abstract

Serum response factor (Srf) is a MADS-box transcription factor that is critical for muscle differentiation. Its function in hematopoiesis has not yet been revealed. Mkl1, a cofactor of Srf, is part of the t(1;22) translocation in acute megakaryoblastic leukemia, and plays a critical role in megakaryopoiesis. To test the role of Srf in megakaryocyte development, we crossed Pf4-Cre mice, which express Cre recombinase in cells committed to the megakaryocytic lineage, to Srf(F/F) mice in which functional Srf is no longer expressed after Cre-mediated excision. Pf4-Cre/Srf(F/F) knockout (KO) mice are born with normal Mendelian frequency, but have significant macrothrombocytopenia with approximately 50% reduction in platelet count. In contrast, the BM has increased number and percentage of CD41(+) megakaryocytes (WT: 0.41% ± 0.06%; KO: 1.92% ± 0.12%) with significantly reduced ploidy. KO mice show significantly increased megakaryocyte progenitors in the BM by FACS analysis and CFU-Mk. Megakaryocytes lacking Srf have abnormal stress fiber and demarcation membrane formation, and platelets lacking Srf have abnormal actin distribution. In vitro and in vivo assays reveal platelet function defects in KO mice. Critical actin cytoskeletal genes are down-regulated in KO megakaryocytes. Thus, Srf is required for normal megakaryocyte maturation and platelet production partly because of regulation of cytoskeletal genes.
View publication
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro F et al. Blood 2009 SEP

Abstract

The role of miRNAs in regulating megakaryocyte differentiation was examined using bipotent K562 human leukemia cells. miR-34a is strongly up-regulated during phorbol ester-induced megakaryocyte differentiation, but not during hemin-induced erythrocyte differentiation. Enforced expression of miR-34a in K562 cells inhibits cell proliferation, induces cell-cycle arrest in G(1) phase, and promotes megakaryocyte differentiation as measured by CD41 induction. miR-34a expression is also up-regulated during thrombopoietin-induced differentiation of CD34(+) hematopoietic precursors, and its enforced expression in these cells significantly increases the number of megakaryocyte colonies. miR-34a directly regulates expression of MYB, facilitating megakaryocyte differentiation, and of CDK4 and CDK6, to inhibit the G(1)/S transition. However, these miR-34a target genes are down-regulated rapidly after inducing megakaryocyte differentiation before miR-34a is induced. This suggests that miR-34a is not responsible for the initial down-regulation but may contribute to maintaining their suppression later on. Previous studies have implicated miR-34a as a tumor suppressor gene whose transcription is activated by p53. However, in p53-null K562 cells, phorbol esters induce miR-34a expression independently of p53 by activating an alternative phorbol ester-responsive promoter to produce a longer pri-miR-34a transcript.
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