Efficiently grow Wnt-independent colorectal tumor organoids using IntestiCult™ OGM Human Basal Medium.
Tumoroids have high predictive value as an in vitro system as they retain the characteristics of the tumor of origin. Use IntestiCult™ OGM Human Basal Medium to provide optimal culture conditions for tumor organoid growth and expansion while maintaining tumor heterogeneity. The Wnt-free formulation ensures that Wnt-dependent, normal, healthy intestinal cells do not contaminate the tumoroid culture.
Tumor organoids generated using IntestiCult™ OGM Human Basal Medium are suitable for a variety of applications, including modeling tumor development and screening drugs for efficacy and toxicity.
Cell Type Cancer Cells and Cell Lines, Epithelial Cells, Intestinal Cells
Species Human
Application Organoid Culture
Brand IntestiCult
Area of Interest Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids
Data Figures
Figure 1. Growth of Cancer-Derived Organoids in IntestiCult™ OGM Human Basal Medium
Organoids were established from colorectal cancer biopsies in published medium1, then switched to IntestiCult™ OGMH (P0), as described below. Cancer-derived organoids demonstrated efficient growth both after establishment in IntestiCult™ OGM Human Basal Medium, as well as after passaging. Data used with permission from Hubrecht Organoid Technology.
Figure 2. IntestiCult™ OGM Human Basal Medium Enables Organoid Growth Across Different Patients
Organoids were established in published medium1 from colorectal tumor biopsy samples, then switched to IntestiCult™ OGM Human Basal Medium after passaging as described above. Organoids were passaged twice in IntestiCult™ OGMH Basal Medium and imaged at the end of the second passage (day 6 - 12). Data used with permission from Hubrecht Organoid Technology.
Figure 3. Colorectal Cancer Organoids Reflect Donor-Specific Colonic Features
Representative confocal images of human colorectal cancer organoids from four donors, cultured using IntestiCult™ OGM Human Basal Medium. Organoids were fixed and stained for Ki67 (green), EPCAM (red) and DAPI (Blue). Donor-specific differences in morphology were observed, including polarization and hollow lumens (A, B), or denser structures, lacking distinct epithelial polarization (C, D). Scale bars = 50 µm.
Figure 4. Colorectal Cancer Organoids Cultured in IntestiCult™OGM Human Basal Medium Are Sensitive to Drug-Induced Toxicity in a Dose-Dependent Manner
Colorectal cancer organoids (CRCs) from 4 donors were treated with increasing concentrations of (A) 5-FU, (B) Flavopiridol, (C) Gefitinib, or (D) Irinotecan, and were compared to organoids from a healthy colon donor for 5 days. Cell viability was assessed on Day 7 using the CellTiter-Glo® Cell Viability Assay (Promega). Error bars = SEM.
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Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov
