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IntestiCult™ 类器官生长基础培养基 (人)

用于建立和维持人结直肠癌类器官的培养基
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产品号 #（选择产品）

产品号 #100-0190_C

用于建立和维持人结直肠癌类器官的培养基

产品优势

  • 使用生理相关的体外体系重现肿瘤特征及其异质性；
  • 通过具有高度代表性的肿瘤类器官预测患者反应；
  • 使用完整的培养基配方获得一致的实验结果；
  • 借助易于操作的形式和优化的实验方案简化流程
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  1. 温和细胞解离试剂
    温和细胞解离试剂
  2. 类器官培养板
    类器官培养板
  3. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES
  4. Y-27632（二盐酸盐）
    Y-27632（二盐酸盐）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用IntestiCult™ 类器官生长基础培养基 (人)高效培养不依赖 Wnt 的结直肠癌类器官。

肿瘤类器官作为一种体外系统具有较高的预测价值，因为它们保留了原发肿瘤的特征。使用IntestiCult™ 类器官生长基础培养基 (人)可为肿瘤类器官的生长和扩增提供最佳培养条件，同时保持肿瘤的异质性。该培养基不含 Wnt，可确保 Wnt 依赖的正常健康肠道细胞不会混入肿瘤类器官的培养。

使用IntestiCult™ 类器官生长基础培养基 (人)生成的肿瘤类器官适用于多种应用，包括模拟肿瘤发展，以及用于药物疗效和毒性的筛选。

关于培养健康的肠道类器官，请使用IntestiCult™ 类器官生长培养基 (人)或IntestiCult™-SF 类器官生长培养基 (人)。关于人肠道类器官的分化，请参考IntestiCult™ 类器官分化培养基 (人)。

欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。

细胞类型
癌细胞及细胞系，上皮细胞，肠道细胞
 
种属
人
 
应用
类器官培养
 
品牌
IntestiCult
 
研究领域
癌症，疾病建模，药物发现和毒理检测，上皮细胞研究，类器官
 

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Data Figures

Figure 1. Growth of Cancer-Derived Organoids in IntestiCult™ OGM Human Basal Medium

Organoids were established from colorectal cancer biopsies in published medium1, then switched to IntestiCult™ OGMH (P0), as described below. Cancer-derived organoids demonstrated efficient growth both after establishment in IntestiCult™ OGM Human Basal Medium, as well as after passaging. Data used with permission from Hubrecht Organoid Technology.

Figure 2. IntestiCult™ OGM Human Basal Medium Enables Organoid Growth Across Different Patients

Organoids were established in published medium1 from colorectal tumor biopsy samples, then switched to IntestiCult™ OGM Human Basal Medium after passaging as described above. Organoids were passaged twice in IntestiCult™ OGMH Basal Medium and imaged at the end of the second passage (day 6 - 12). Data used with permission from Hubrecht Organoid Technology.

Confocal images of human colorectal cancer organoids stained with Ki67, EPCAM, and DAPI.

Figure 3. Colorectal Cancer Organoids Reflect Donor-Specific Colonic Features

Representative confocal images of human colorectal cancer organoids from four donors, cultured using IntestiCult™ OGM Human Basal Medium. Organoids were fixed and stained for Ki67 (green), EPCAM (red) and DAPI (Blue). Donor-specific differences in morphology were observed, including polarization and hollow lumens (A, B), or denser structures, lacking distinct epithelial polarization (C, D). Scale bars = 50 µm.

IC50 toxicity graphs demonstrating the response of colorectal cancer organoids to various cancer drugs.

Figure 4. Colorectal Cancer Organoids Cultured in IntestiCult™OGM Human Basal Medium Are Sensitive to Drug-Induced Toxicity in a Dose-Dependent Manner

Colorectal cancer organoids (CRCs) from 4 donors were treated with increasing concentrations of (A) 5-FU, (B) Flavopiridol, (C) Gefitinib, or (D) Irinotecan, and were compared to organoids from a healthy colon donor for 5 days. Cell viability was assessed on Day 7 using the CellTiter-Glo® Cell Viability Assay (Promega). Error bars = SEM.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
IntestiCult™ OGM Human Basal Medium
Catalog #
100-0190
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
IntestiCult™ OGM Human Basal Medium
Catalog #
100-0190
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (20)

Intestinal Organoids
Brochure
Intestinal Organoids
How to Isolate Human Colonic Crypts from Intestinal Biopsies
Protocol
How to Isolate Human Colonic Crypts from Intestinal Biopsies
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
Protocol
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
How to Generate Human Intestinal Organoid-Derived Monolayers Using IntestiCult™
Protocol
How to Generate Human Intestinal Organoid-Derived Monolayers Using IntestiCult™
How to Thaw Intestinal Organoids
Protocol
How to Thaw Intestinal Organoids
Toxicity Testing for Drug Development Using Human Intestinal Organoids and IntestiCult™
Protocol
Toxicity Testing for Drug Development Using Human Intestinal Organoids and IntestiCult™
Culturing Intestinal Cancer-Derived Organoids
Protocol
Culturing Intestinal Cancer-Derived Organoids
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
47:49
Webinar
Advances in GI Organoid Applications: Drug Discovery and Immunotherapy
STEMCELL Seminar: Functional Applications of Intestinal Organoids
21:26
Webinar
STEMCELL Seminar: Functional Applications of Intestinal Organoids
Patient-Derived Organoids for Drug Screening and Development
50:25
Webinar
Patient-Derived Organoids for Drug Screening and Development
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
1:03:40
Webinar
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
17:32
Webinar
Intestinal Organoids: Improved Tools and In Vitro Models for Drug Development
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Intestinal Organoid Culture
Mini Review
Intestinal Organoid Culture
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Scientific Poster
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
E-Book
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
Human Intestinal Organoid Course
On-Demand Training
Human Intestinal Organoid Course

更多信息

更多信息
Species Human
Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov
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