产品号 #78067_C
转化生长因子β1
将这种高质量的人重组转化生长因子β1 (TGF-β1)添加到您的细胞培养工作流程中。经验证,其内毒素水平≤0.2 EU/μg(采用鲎试剂法测定),可确保在多种应用中安心使用。作为 TGF-β 超家族的一员,TGF-β1 可调节多种细胞表型。TGF-β1 与 I 型和 II 型丝氨酸-苏氨酸激酶受体结合,并通过 SMAD2/3 蛋白激活信号转导。
TGF-β1可用于:
hPSC维持培养
将 TGF-β1 与碱性成纤维细胞生长因子 (bFGF) 联合使用,以支持未分化人胚胎干细胞 (ES) 和诱导性多能干细胞 (iPS) 的培养 (Amit et al. Biol Reprod, 2004)。
外源性细胞信号转导
调节多种功能,包括细胞增殖、分化和代谢 (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997)。
免疫细胞稳态
调节B细胞应答并介导对T细胞和中性粒细胞的免疫抑制作用 (Letterio & Roberts Annu Rev Immunol, 1998)。
亚型
细胞因子,生长因子
细胞类型
B 细胞,间充质干/祖细胞,单核细胞,髓系细胞,神经干/祖细胞,多能干细胞,T 细胞,调节性细胞
种属
人
研究领域
免疫,神经科学,干细胞生物学
纯度
≥ 95 %
Figure 1. Human Recombinant TGF-beta 1 Is Bioactive
The biological activity of Human Recombinant TGF-beta 1 was tested by the ability to inhibit IL-4-induced HT-2 cell proliferation. Cell proliferation was measured after 2 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.17 ng/mL.
Figure 2. Human Recombinant TGF-beta 1 Has High Purity
1 μg of Human Recombinant TGF-beta 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
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Species | Human |
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Purity | ≥ 95% |
免疫磁珠负选人CD4+ T细胞
白介素10
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