重组人TGF - β 1

转化生长因子β1

产品号 #(选择产品)

产品号 #78067_C

转化生长因子β1

总览

将这种高质量的人重组转化生长因子β1 (TGF-β1)添加到您的细胞培养工作流程中。经验证,其内毒素水平≤0.2 EU/μg(采用鲎试剂法测定),可确保在多种应用中安心使用。作为 TGF-β 超家族的一员,TGF-β1 可调节多种细胞表型。TGF-β1 与 I 型和 II 型丝氨酸-苏氨酸激酶受体结合,并通过 SMAD2/3 蛋白激活信号转导。

TGF-β1可用于:
hPSC维持培养
将 TGF-β1 与碱性成纤维细胞生长因子 (bFGF) 联合使用,以支持未分化人胚胎干细胞 (ES) 和诱导性多能干细胞 (iPS) 的培养 (Amit et al. Biol Reprod, 2004)。
外源性细胞信号转导
调节多种功能,包括细胞增殖、分化和代谢 (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997)。
免疫细胞稳态
调节B细胞应答并介导对T细胞和中性粒细胞的免疫抑制作用 (Letterio & Roberts Annu Rev Immunol, 1998)。

亚型
细胞因子,生长因子
 
细胞类型
B 细胞,间充质干/祖细胞,单核细胞,髓系细胞,神经干/祖细胞,多能干细胞,T 细胞,调节性细胞
 
种属

 
研究领域
免疫,神经科学,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

Figure 1. Human Recombinant TGF-beta 1 Is Bioactive

The biological activity of Human Recombinant TGF-beta 1 was tested by the ability to inhibit IL-4-induced HT-2 cell proliferation. Cell proliferation was measured after 2 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.17 ng/mL.

Figure 2. Human Recombinant TGF-beta 1 Has High Purity

1 μg of Human Recombinant TGF-beta 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78067.1, 78067, 78067.2, 78067.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78067.1, 78067, 78067.2, 78067.3
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Purity ≥ 95%
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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