产品号 #100-1797_C
Transforming growth factor beta 1, animal component-free
概述
Transforming growth factor beta 1 (TGF-β1) is a member of the TGF-β superfamily and regulates diverse cellular phenotypes. With a verified endotoxin level of ≤ 0.1 EU/μg, measured using the LAL method, confidently use TGF-β1 across multiple applications. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins, thus regulating a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué; McDowell et al.). During human embryogenesis, TGF-β1 is expressed by endothelial and hematopoietic tissues and acts as an endogenous autocrine growth regulator (Gatherer et al.). Together with basic fibroblast growth factor (bFGF), TGF-β1 supports the culture of undifferentiated human embryonic stem cells and induced pluripotent stem cells (Amit et al.). Once activated, TGF-β1 helps maintain immune homeostasis by modulating B cell response and mediating immunosuppressive effects on T cells and neutrophils (Letterio & Roberts). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
Cartilage-inducing factor, Differentiation inhibiting factor, LAP, Latency-associated peptide, TGFB1, TGF-beta-1, Transforming growth factor β 1
Cell Type
B Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Myeloid Cells, Neural Stem and Progenitor Cells, Pluripotent Stem Cells, T Cells, Regulatory
Species
Human, Other
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98% by SDS-PAGE
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant TGF-beta 1, ACF
(A) The biological activity of Human Recombinant TGF-beta 1, ACF was tested using a luciferase reporter assay in transiently transfected HEK293T cells. Firefly luciferase activity was normalized to control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which TGF beta 1 response is at 50% of maximum. The EC50 in the above example is 1.54 pM (~0.04 ng/mL).
(B) 7 μg of Human Recombinant TGF-beta 1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TGF-beta 1, ACF has a predicted molecular mass of 24 kDa (dimer).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant TGF-beta 1, ACF Catalog #
100-1798 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant TGF-beta 1, ACF Catalog #
100-1798 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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