产品号 #78062_C
干细胞因子
总览
干细胞因子 (SCF) 是一种早期作用的细胞因子，在胚胎和成人造血的调控中起着关键作用。SCF 促进细胞存活、增殖、分化、粘附以及在造血系统多个层面的功能性激活。SCF 与其他细胞因子（例如血小板生成素和 Flt3/Flk-2 配体）一起，常用于促进培养的原始造血干细胞和多能祖细胞的扩增 (Martin et al.; Kent et al.)。SCF 与多种生长因子（包括 IL-2、IL-3、IL-6、IL-7、G-CSF 和促红细胞生成素）协同作用，促进髓系和红系祖细胞以及部分淋巴系祖细胞的增殖和分化 (Broudy)。SCF 也是肥大细胞和嗜酸性粒细胞的主要生长和活化因子。
SCF 有两种生物活性剪接形式：可溶性剪接体和跨膜型剪接体。与其受体（c-Kit 酪氨酸激酶受体；CD117）结合后，SCF 可激活 PI3K、JAK/STAT 和 MAPK 通路。据报道，SCF 和 c-Kit 信号转导在色素沉着、生育力、血管生成、通过 c-Kit 阳性的 Cajal 间质细胞促进肠道运动以及神经干细胞和祖细胞向脑损伤部位迁移方面发挥重要作用。
亚型
细胞因子
细胞类型
造血干/祖细胞，中胚层，PSC衍生，多能干细胞
种属
人
研究领域
干细胞生物学
纯度
≥ 97 %
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. Nature communications 2014 jul
Abstract
Advancing pluripotent stem cell technologies for modelling haematopoietic stem cell development and blood therapies requires identifying key regulators of haematopoietic commitment from human pluripotent stem cells (hPSCs). Here, by screening the effect of 27 candidate factors, we reveal two groups of transcriptional regulators capable of inducing distinct haematopoietic programs from hPSCs: pan-myeloid (ETV2 and GATA2) and erythro-megakaryocytic (GATA2 and TAL1). In both cases, these transcription factors directly convert hPSCs to endothelium, which subsequently transform into blood cells with pan-myeloid or erythro-megakaryocytic potential. These data demonstrate that two distinct genetic programs regulate the haematopoietic development from hPSCs and that both of these programs specify hPSCs directly to haemogenic endothelial cells. In addition, this study provides a novel method for the efficient induction of blood and endothelial cells from hPSCs via the overexpression of modified mRNA for the selected transcription factors.
更多信息
| Species | Human |
|---|---|
| Purity | ≥ 97% |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
