产品号 #78215_C
Receptor activator of nuclear factor kappa-B ligand, animal component-free
概述
Receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily (Anderson et al.). Cytokines in the TNF superfamily are involved in a variety of long-term cellular activities such as differentiation, proliferation, and cell death (MacEwan). RANKL is a type II homotrimeric transmembrane protein expressed in both a membrane-bound and secreted form (Ikeda et al.). RANKL binds to the receptor activator of nuclear factor kappa-B (RANK). Upon binding to its receptor, RANKL activates the AKT signaling pathway (Moon et al.). Osteoprotegerin (OPG) may also bind RANKL, and this binding competes with RANKL-RANK binding (Lacey et al.). RANKL is involved in osteoclastogenesis (Lacey et al.; Yasuda et al.) and T cell activation (Wong et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
<p>CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoclast differentiation factor, soluble Receptor activator of NF-κB ligand, sOdf, TNF-related activation-induced cytokine, TNFSF11, TNF superfamily member 11, TNLG6B, Tumor necrosis factor superfamily member 11, TRA</p>
Cell Type
Airway Cells, Cancer Cells and Cell Lines, Dendritic Cells, Intestinal Cells, Mammary Cells, Osteoblasts, Other, Pancreatic Cells, Prostate Cells, T Cells
Species
Human
Area of Interest
Cancer, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant RANKL, ACF was tested by its ability to induce production of embryonic alkaline phosphatase in RAW-Blue™ macrophage reporter cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 38.8 ng/mL. (B) Human Recombinant RANKL, ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant RANKL, ACF has a predicted molecular mass of 19.7 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1 Product Name
Human Recombinant RANKL, ACF Catalog #
78215, 78215.1, 78215.2 Lot #
1000154450 or lower Language
English Document Type
Product Information Sheet 2 Product Name
Human Recombinant RANKL, ACF Catalog #
78215, 78215.1, 78215.2 Lot #
1000154451 or higher Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant RANKL, ACF Catalog #
78215, 78215.1, 78215.2 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Airway Epithelial Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Intestinal Epithelial Cell Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Prostate Epithelial Cell Research
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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