产品号 #100-2064_C
R-spondin-3, animal component-free
概述
R-Spondin-3 is a member of thrombospondin type 1 repeat (TSR-1) superfamily that is involved in the canonical Wnt/β-catenin signaling pathway (de Lau et al.). R-spondin proteins are characterized by two furin-like repeats at the amino terminus and thrombospondin domain located near the carboxyl terminus (de Lau et al.). R-spondin-3 expression is associated with ovarian cancer (Gu et al.), prostate cancer (Mesci et al.), and differentiation of intestinal epithelial cells in diabetes mellitus (Shan et al.). In a transgenic mouse model, the expression of R-Spondin-3 induces the expansion of Lgr5+ stem cells, Paneth cells, and Lgr4+ cells, promoting the intestinal stem cell compartment (Hilkens et al.). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
Cristin 1, hRspo3, PWTSR, R-Spondin 3, Roof plate-specific spondin-3, RSPO3, RSpondin 3, Thrombospondin type-1 domain-containing protein 2, THSD2
Cell Type
Epithelial Cells
Species
Human
Area of Interest
Organoids, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98% by SDS-PAGE
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant R-spondin-3, ACF
(A) The biological activity of Human Recombinant R-Spondin-3, ACF was tested by its ability to induce Wnt-ß catenin signaling in HEK239T cells using a luciferase reporter assay. Firefly luciferase activity was normalized to control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which Wnt-ß catenin response is at 50% of maximum. The EC50 in the above example is 0.3 nM (5 ng/mL).
(B) 7 μg of Human Recombinant R-spondin-3, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant R-spondin-3, ACF has a predicted molecular mass of 17 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Lot #
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Document Type
Product Information Sheet Product Name
Human Recombinant R-spondin-3, ACF Catalog #
100-2065 Lot #
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English Document Type
Safety Data Sheet Product Name
Human Recombinant R-spondin-3, ACF Catalog #
100-2065 Lot #
All Language
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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