Human Recombinant Neurotrophin-3 (NT-3)

概述

神经营养因子-3 （NT-3）是一种神经营养因子，也是神经生长因子（NGF）蛋白家族的成员，该蛋白家族包括神经元生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子-4/5。NT-3通过其转导受体酪氨酸激酶TrkC发出多种营养效应信号。NT-3可以促进神经元的存活、发育和分化，并调节外周和中枢神经系统中几种突触的递质释放（Chalazonitis 1996）。NT-3已被证明在肠道神经元的整体发育中具有重要作用，而肠道神经元对肠道蠕动至关重要（Chalazonitis 2004）。对大鼠的研究表明NT-3在背柱轴突再生中的潜力（Bradbury等人）。NT-3被证明可以保护神经元免受淀粉样蛋白-β的毒性（Lesne等人）。NT-3已应用于神经分化方案，从神经嵴干细胞（Menendez等人）和从人胚胎干细胞（ES）和诱导多能干细胞（iPS）细胞（Douvaras等人）中产生β-微管蛋白III+周围神经元。

Subtype
Cytokines

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons

Species
Human, Mouse

Area of Interest
Neuroscience

Purity
≥ 95%

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Data Figures

(A) The biological activity of Human/Mouse Recombinant NT-3 was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6). Cell proliferation was measured using a colorimetric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 9.3 ng/mL. For a representative bioactivity plot of C6 cell proliferation, contact us at techsupport@stemcell.com. (B) 1 μg of Human/Mouse Recombinant NT-3 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human/Mouse Recombinant NT-3 is predicted to be a non-covalently linked homodimer, with each dimer having a molecular mass of 13.8 kDa for a predicted total molecular weight of 27.5 kDa.