产品号 #100-1731_C
Noggin, animal component-free
概述
Use noggin to support the maintenance of undifferentiated human embryonic stem cells in vitro, and to prevent spontaneous differentiation in the short term (Chaturvedi et al. Cell Prolif, 2009). Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al. Int J Biochem Cell Biol, 2011), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al. Stem Cells, 2008).
Subtype
Cytokines
Alternative Names
NOG, SYM1, SYNS1
Cell Type
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98%
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant Noggin (E. coli-expressed), ACF
(A) The biological activity of Human Recombinant Noggin (E. coli-expressed), ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells. Inhibition of BMP-2 was measured using a firefly luciferase reporter assay. Cells were treated with a serial dilution of Noggin and standard concentration of BMP-2 for 6 hours. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.5 nM (70.1 ng/mL). (B) 7 μg of Human Recombinant Noggin (E. coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Noggin has a predicted molecular mass of 23 kDa in reducing conditions.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Noggin (E. coli-expressed), ACF Catalog #
100-1731, 100-1732 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Noggin (E. coli-expressed), ACF Catalog #
100-1731 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Noggin (E. coli-expressed), ACF Catalog #
100-1732 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Resources and Publications
Educational Materials (3)
Request Pricing
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
欢迎您关注STEMCELL Technologies的微信公众平台
扫描二维码或搜索微信号STEMCELLTech，即可关注我们的微信平台，第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题，欢迎发消息给STEMCELLTech微信公众平台，或与我们通过电话/邮件联系：400 885 9050 INFO.CN@STEMCELL.COM。