重组人M-CSF, ACF

巨噬细胞集落刺激因子,不含动物成分

产品号 #(选择产品)

产品号 #78150_C

巨噬细胞集落刺激因子,不含动物成分

总览

巨噬细胞集落刺激因子(M-CSF)是一种同源二聚体糖蛋白生长因子,可调节髓系造血祖细胞向单核吞噬细胞谱系的增殖和分化,包括单核细胞、巨噬细胞和破骨细胞。在大多数组织中,M-CSF是组织常驻巨噬细胞发育的关键因子(Ginhoux & Jung)。它是单核细胞和巨噬细胞成熟和激活所必需的,并与其他刺激如IFN-γ、LPS和IL-4一起调节炎症反应(Murray等)。M-CSF也是破骨细胞骨吸收所必需的,并参与胎盘、乳腺和脑的发育和调节。M-CSF由单核细胞、成纤维细胞、破骨细胞、基质细胞、内皮细胞和肿瘤细胞产生(Chockalingam & Ghosh)。


M-CSF通过c-fms原癌基因编码的受体酪氨酸激酶(CSF-1R或M-CSF-R)信号传导发挥其生物学作用(Hamilton)。CSF-1R与其他生长因子受体具有相似的结构特征,包括干细胞因子(SCF)受体、血小板衍生生长因子受体(PDGF-R)和Flt3/Flk-2受体酪氨酸激酶。CSF-1R与M-CSF结合后的刺激可激活MAPK、PI3K和PLCγ信号通路(Chockalingam & Ghosh)。人和小鼠M-CSF序列在核苷酸和氨基酸水平上高度保守(同源性80%;DeLamarter等)。本产品不含动物成分。

亚型
细胞因子
 
细胞类型
树突状细胞(DCs),造血干/祖细胞,中胚层,PSC衍生,单核细胞,髓系细胞,神经元
 
种属

 
研究领域
免疫,干细胞生物学
 
制剂类别
Animal Component-Free
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Human Recombinant M-CSF, ACF was tested by its ability to promote the proliferation of NFS-60 cells. Cell proliferation was measured after 62 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 3 ng/mL. (B) 1 μg of Human Recombinant M-CSF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78150.1, 78150, 78150.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78150.1, 78150, 78150.2
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Formulation Category Animal Component-Free
Purity ≥ 95%
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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