产品号 #78194_C
Interleukin 3
概述
Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).
Subtype
Cytokines
Cell Type
Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, NK Cells, T Cells
Species
Human
Area of Interest
Immunology
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant IL-3 (CHO-expressed) was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.1 ng/mL. (B) 2 μg of Human Recombinant IL-3 (CHO-expressed) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-3 (CHO-expressed) has a predicted molecular mass of 15.1 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant IL-3 (CHO-expressed) Catalog #
78194, 78194.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant IL-3 (CHO-expressed) Catalog #
78194, 78194.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Resources and Publications
Educational Materials (4)
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