产品号 #78013_C
Dickkopf-related protein 1
概述
Dickkopf-related protein 1 (DKK-1) is a member of the Dickkopf family and is a secreted protein that inhibits the canonical WNT pathway by competitive binding to low-density lipoprotein receptors (LRP)-5 and 6 with high affinity, thereby decreasing β-catenin protein stability (Niehrs). DKK-1 regulates embryonic development and contains two conserved cysteine-rich domains separated by a linker region and an N-terminal signal peptide (Krupnik et al.; Lieven et al.). A family of human DKK-related genes composed of DKK-1, DKK-2, DKK-3, and DKK-4 have been characterized together with a unique DKK-3 related protein termed Soggy (Krupnik et al.). DKK-1 has been shown to support the generation of myeloid-derived suppressor cells (MDSCs) and thus is a negative regulator of antitumor immune responses (D’Amico et al.). DKK-1 from thrombocytes is an important regulator of leukocyte infiltration and induces Th2 cell polarization and potentiates Th2 cell cytokine expression (Chae et al.). DKK-1 has also been shown to drive cardiac and retinal differentiation from induced pluripotent stem (iPS) cells (Lian et al.). Protein contains a His-residue tag at the carboxyl end of the polypeptide chain.
Subtype
Cytokines
Alternative Names
Dickkopf-1, hDkk-1, SK, Dickkopf WNT signaling pathway inhibitor 1
Cell Type
Endoderm, PSC-Derived, Myeloid Cells, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology
Molecular Weight
17 - 22 kDa
Purity
≥ 95 %
Data Figures
(A) The biological activity of Human Recombinant DKK-1 was tested by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T1/2 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the above example is less than 4 μg/mL.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant DKK-1 Catalog #
78208.1, 78208.2 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant DKK-1 Catalog #
78208.1, 78208.2 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Resources and Publications
Educational Materials (2)
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