产品号 #78162_C
Cluster of differentiation 40 ligand
概述
CD40 ligand is a type II transmembrane glycoprotein that belongs to the tumor necrosis factor (TNF) superfamily (Quezada et al.). CD40 ligand forms a bioactive homotrimer that exists as both soluble and membrane-bound forms (Khandekar et al.). CD40 ligand is expressed on T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. Its receptor, CD40, is expressed on B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (van Kooten & Banchereau). Binding of CD40 ligand to CD40 stimulates B cell proliferation, immunoglobulin class switching, antibody secretion, and T cell-dependent humoral responses. Dysregulation of CD40 ligand contributes to immune deficiency in HIV and AIDS (Rickert et al.). CD40 ligand has also been linked to the pathology of atherosclerosis, atherothrombosis, and restenosis (Hassan et al.).
Subtype
Cytokines
Cell Type
B Cells, Dendritic Cells, Endothelial Cells, Granulocytes and Subsets, Monocytes, T Cells
Species
Human
Area of Interest
Immunology
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant CD40 Ligand was tested by its ability to induce alkaline phosphatase activity in HEKBlue ™ CD40L responsive indicator cells. Alkaline phosphatase activity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the ligand at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is 16.7 ng/mL. (B) 1 μg of Human Recombinant CD40 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant CD40 Ligand has a predicted molecular mass of 16.3 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant CD40 Ligand Catalog #
78162, 78162.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant CD40 Ligand Catalog #
78162, 78162.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Monocyte Research
Resources and Publications
Educational Materials (3)
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