For detection and measurement of human RANTES (CCL5)
The Human RANTES (CCL5) ELISA Kit is designed for the quantitative detection and measurement of human RANTES (regulated upon activation, normal T cell expressed and secreted), also known as CCL5, in biological fluids such as serum, plasma, and cell culture supernatants. RANTES is secreted by T cells, fibroblasts, macrophages, epithelial cells, and endothelial cells induced by inflammatory stimuli such as IFN-γ, TNFα, or IL-1. RANTES binds to three different chemokine receptors: CCR1, CCR3 and CCR5. The CCR5 is the main co-receptor for macrophage tropic strains of HIV-1 and HIV-2 and acts as an HIV-suppressor when secreted by CD8+ Tcells. RANTES also acts as an inflammatory mediator inducing lymphocyte and monocyte migration that suppresses pro-malignancy activities in breast cancer.
The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human RANTES. The captured RANTES is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human RANTES in the sample.
Subtype
Complete Kits
Cell Type
Endothelial Cells, Epithelial Cells, Fibroblasts, Macrophages, T Cells
Species
Human
Application
ELISA
Area of Interest
Immunology, Infectious Diseases
Figure 1. Representative Standard Curve Of Human RANTES (CCL5)
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 3.13 - 200 pg/mL. The lower limit of detection (LLD) of this assay is 0.50 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 96 - 102%, 96 - 104%, or 87 - 98% was determined by spiking defined amounts of analyte standard into cell culture supernatant, serum, or plasma samples, respectively, in repeated experiments. The intra-assay precision of this assay is 6.0% (CV). The Inter-assay precision of this assay is 2.5% (CV).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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