产品号 #100-1147_C
用于检测和测量人体RANTES (CCL5)
人 RANTES (CCL5) ELISA 试剂盒用于定量检测和测定血清、血浆和细胞培养上清液等生物体液中人 RANTES(活化后调节,正常 T 细胞表达和分泌),也称为 CCL5。RANTES 由 T 细胞、成纤维细胞、巨噬细胞、上皮细胞和内皮细胞分泌,这些细胞受 IFN-γ、TNFα 或 IL-1 等炎症刺激诱导。RANTES 与三种不同的趋化因子受体结合:CCR1、CCR3 和 CCR5。CCR5 是 HIV-1 和 HIV-2 巨噬细胞嗜性毒株的主要辅助受体,由 CD8+ T 细胞分泌时可作为 HIV 抑制剂。RANTES 还作为炎症介质,诱导淋巴细胞和单核细胞迁移,从而抑制乳腺癌中的促癌活性。
该检测基于夹心ELISA方法,将样品加入预先包被有针对人类RANTES的捕获抗体的ELISA可拆酶标板。通过添加辣根过氧化物酶(HRP)偶联的检测抗体来检测捕获的RANTES。添加显色酶底物3,3',5,5'-四甲基联苯胺 (TMB)后,显色产物的强度与样品中人类RANTES的浓度成正比。
亚型
完整试剂盒
细胞类型
内皮细胞,上皮细胞,成纤维细胞,巨噬细胞,T 细胞
种属
人
应用
ELISAs
研究领域
免疫,感染性疾病(传染病)
Figure 1. Representative Standard Curve Of Human RANTES (CCL5)
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 3.13 - 200 pg/mL. The lower limit of detection (LLD) of this assay is 0.50 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 96 - 102%, 96 - 104%, or 87 - 98% was determined by spiking defined amounts of analyte standard into cell culture supernatant, serum, or plasma samples, respectively, in repeated experiments. The intra-assay precision of this assay is 6.0% (CV). The Inter-assay precision of this assay is 2.5% (CV).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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透明聚苯乙烯圆底未经组织培养处理的带盖微孔板
激活后调节正常T细胞表达和分泌因子
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