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Human Peripheral Blood Leukopak, Frozen

Primary human cells, frozen
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产品号 #200-0130_C

Primary human cells, frozen

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  • Allows for the isolation of large quantities of Mononuclear Cells from a single donor.

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Human Peripheral Blood Leukopak, Frozen
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

Start experiments when you want to with large numbers of leukocytes, including peripheral blood mononuclear cells (PBMCs). As a cryopreserved product, frozen leukopaks provide samples from specific donors that can be processed when the end-user is ready. We help you get the cells you need with personalized service, custom products, flexible delivery times, and multiple size options.

Each frozen leukopak is a highly concentrated, low-volume apheresis collection from a single, normal donor, using Institutional Review Board (IRB)-approved consent forms and protocols. Each full-sized leukopak is produced from ~2 - 3x blood volumes using the Spectra Optia® Apheresis System, with acid-citrate-dextrose solution A (ACDA) as the anticoagulant. The collected product is then cryopreserved in a controlled-rate freezer. High-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse Frequently Asked Questions (FAQs) on Primary Cells.

Subtype
Frozen
 
Cell Type
Leukopaks, Mononuclear Cells
 
Species
Human
 
Cell and Tissue Source
Peripheral Blood
 
Donor Status
Normal
 

Data Figures

Figure 1. Typical Flow Cytometric Analysis Profile of a Leukopak Before Cryopreservation

Representative gating strategy for flow cytometric immunophenotyping of the different cell subpopulations present in a leukopak product prior to cryopreservation. The CD45 plot was gated on viable single cells while all other plots were gated on viable CD45+ single cells. In the above example, the cell frequencies (as a percentage of the overall leukopak product) are as follows: monocytes (CD14+) 19.9%, B cells (CD19+) 13.9%, NK cells (CD3-CD56+) 6.3%, Helper T cells (CD3+CD4+) 28.5%, and Cytotoxic T cells (CD3+CD8+) 13.5%.

Figure 2. Mean Percentages of Cell Subpopulations in a Leukopak

Representative chart showing the average frequencies of major immune subsets in leukopak products, as measured by flow cytometry prior to cryopreservation. Values shown are mean percentages of total viable leukocytes present in a leukopak (n ≥ 30).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Peripheral Blood Leukopak, Frozen
Catalog #
200-0132, 200-0131, 200-0130, 200-0470
Lot #
All
Language
English
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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