产品号 #200-0371_C
Primary human cells, frozen
概述
Streamline your assays with ready-to-use, ethically sourced, animal component-free-cultured, primary human mature dendritic cells (DCs). With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need.
Peripheral blood (PB) monocytes are isolated by negative immunomagnetic selection and cultured for 5 days in serum- and animal component-free ImmunoCult™-ACF Dendritic Cell Medium (Catalog #10986) and ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (Catalog #10988), which contains a combination of recombinant human cytokines formulated to support the differentiation of human monocytes to immature DCs (iDCs). Next, the iDCs are matured for 2 days with ImmunoCult™ Dendritic Cell Maturation Supplement (Catalog #10989).
Cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
CryoStor® CS10
Subtype
Frozen
Cell Type
Dendritic Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Area of Interest
Disease Modeling, Stem Cell Biology, Cell Therapy Development
Donor Status
Normal
Purity
≥ 90% CD11c+, ≥ 90% MHC class II+, ≥ 90% CD83+, ≥ 90% CD80+, and ≤ 5% CD14high by flow cytometry
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (5)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
