Primary human cells, frozen
Choose ready-to-use, ethically sourced, primary human CD19+CD27+ memory B cells with or without donor-matched CD19+CD27- naïve B cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.
CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic separation to isolate particle-free CD27+ cells, and unwanted cells are subsequently targeted for immunomagnetic depletion. Donor-matched CD19+CD27- naïve B cells are isolated using negative immunomagnetic separation. Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols, and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant.
Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories. For more information, browse frequently asked questions (FAQs) on primary cells.
Note: If you only require CD19+CD27- naïve B cells, and not CD19+CD27+ memory B cells with donor-matched CD19+CD27- naïve B cells, we recommend using Human Peripheral Blood CD19+CD27- Naïve B Cells, Frozen (Catalog #70032).
Contains
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CryoStor® CS10
Subtype
Frozen
Cell Type
B Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal
Purity
≥ 85% CD19+CD27+ by flow cytometry
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
