The Human pan Interferon-alpha (IFN-α) ELISA Kit is designed for the quantitative detection and measurement of human IFN-α in biological fluids such as serum, plasma, and cell culture supernatants. IFN-α is primarily produced by macrophages resulting from viral infections and activates anti-viral and immunomodulatory activities in target cells. IFN-α is secreted as multiple subtypes; this kit is specific for subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype Complete Kits
Cell Type Dendritic Cells, Macrophages
Species Human
Application ELISA
Area of Interest Immunology
Data Figures
Representative Standard Curve
• Reportable range: 3.2 - 316 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. • Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 95/566. • Recovery: A mid-curve recovery of 92 - 123% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 10.0% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
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