产品号 #100-1794_C
Follistatin-resistant Activin A, animal component-free
概述
Follistatin-resistant activin A (FRACTA) is a modified version of Activin A that has been engineered to reduce binding to follistatin, the natural inhibitor of Activin A. Activin A is a member of the transforming growth factor beta (TGF-β) family of proteins produced by many cell types throughout development (Gurdon et al.). It is a disulfide-linked homodimer (two beta-A chains) that binds to heteromeric complexes of a type I (Act RI-A and Act RI-B) and a type II (Act RII-A and Act RII-B) serine-threonine kinase receptor (Attisano et al.). Activins primarily signal through SMAD2/3 proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (McDowell et al.). Activin A signaling is regulated by binding of follistatin, which blocks the type-II receptor binding site (Harrington et al.). Activin A maintains the undifferentiated state of human embryonic stem cells (James et al.; Xiao et al.) and also facilitates differentiation of human embryonic stem cells into definitive endoderm (D’Amour et al.). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
Activin beta-A chain, EDF, Erythroid differentiation protein, FRACTA, FRP, FSH-releasing protein, INHBA, Inhibin betaA chain, Inhibin beta-1
Cell Type
Endoderm, PSC-Derived, Mesoderm, PSC-Derived, Other, Pluripotent Stem Cells
Species
Human, Mouse, Rat
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98% by SDS-PAGE
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF
(A) The biological activity of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF was tested using an activin-responsive luciferase reporter assay in HEK293T cells. Firefly luciferase activity is normalized to control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which activin response is at 50% of maximum. The EC50 in the above example is 8.79 pM (0.23 ng/mL).
(B) 1 μg of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF has a predicted molecular mass of 26 kDa (dimer).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Mouse Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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