产品号 #100-1139_C
用于人白细胞介素-7的检测和测定
人白细胞介素7 (IL-7)酶联免疫吸附测定试剂盒用于细胞培养上清中人白细胞介素7的定量检测和测定。IL-7是一种造血生长因子,由骨髓和胸腺的基质细胞分泌。它通过IL-7R发出信号,刺激造血干细胞向淋巴样祖细胞分化。IL-7对T细胞稳态很重要,并促进naïve和记忆T细胞的细胞存活和增殖。
该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的人IL-7特异性抗体。捕获的IL-7通过添加辣根过氧化物酶(HRP)偶联检测抗体进行检测。添加显色酶底物3,3 ‘ 5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中人IL-7的浓度成正比。
Subtype
Complete Kits
Cell Type
Hematopoietic Stem and Progenitor Cells, T Cells
Species
Human
Application
ELISA
Area of Interest
Immunology, Infectious Diseases
Figure 1. Representative Standard Curve Of Human IL-7
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 5.47 - 350 pg/mL. The lower limit of detection (LLD) of this assay is 2.07 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 98 - 108% was determined by spiking defined amounts of analyte standard into cell culture supernatant in repeated experiments. The intra-assay precision of this assay is 4.3% (CV). The Inter-assay precision of this assay is 6.2% (CV).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Species | Human |
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透明聚苯乙烯圆底未经组织培养处理的带盖微孔板
白介素7
白细胞介素7,不含动物成分
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