人IL-7酶联免疫吸附测定试剂盒

用于人白细胞介素-7的检测和测定

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产品号 #100-1139_C

用于人白细胞介素-7的检测和测定

产品组分包括

人IL-7酶联免疫吸附测定试剂盒

人白细胞介素-7酶联免疫吸附试验板
人IL-7 HRP检测抗体，1瓶
人白细胞介素-7标准品，1瓶
洗涤缓冲液（20X）， 30ml
稀释缓冲液（20X）， 12.5 mL
标准稀释缓冲液，2ml
过氧化物溶液，12.5 mL
TMB底物，12.5 mL
停止液，11毫升

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概述

人白细胞介素7 （IL-7）酶联免疫吸附测定试剂盒用于细胞培养上清中人白细胞介素7的定量检测和测定。IL-7是一种造血生长因子，由骨髓和胸腺的基质细胞分泌。它通过IL-7R发出信号，刺激造血干细胞向淋巴样祖细胞分化。IL-7对T细胞稳态很重要，并促进naïve和记忆T细胞的细胞存活和增殖。

该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的人IL-7特异性抗体。捕获的IL-7通过添加辣根过氧化物酶（HRP）偶联检测抗体进行检测。添加显色酶底物3,3 ‘ 5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中人IL-7的浓度成正比。

Data Figures

Figure 1. Representative Standard Curve Of Human IL-7

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 5.47 - 350 pg/mL. The lower limit of detection (LLD) of this assay is 2.07 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 98 - 108% was determined by spiking defined amounts of analyte standard into cell culture supernatant in repeated experiments. The intra-assay precision of this assay is 4.3% (CV). The Inter-assay precision of this assay is 6.2% (CV).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Product Information Sheet

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Human IL-7 ELISA Kit

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