For detection and measurement of human interleukin-6
The Human Interleukin 6 (IL-6) ELISA kit is designed for the quantitative detection and measurement of IL-6 in biological fluids such as serum and cell culture supernatants. IL-6 is primarily produced by macrophages, fibroblasts, activated T cells, and endothelial cells. Signaling through the IL-6R receptor, IL-6 activates glycoprotein 130 and subsequent downstream signaling. It is an important growth factor for plasmacytoma and myeloma and enhances B cell proliferation. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression and has been defined as an essential cytokine in directing the transition from innate to acquired immunity.
The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human IL-6. The captured IL-6 is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human IL-6 in the sample.
Subtype
Complete Kits
Cell Type
Endothelial Cells, Fibroblasts, Macrophages, T Cells
Species
Human
Application
ELISA
Area of Interest
Immunology, Infectious Diseases
Figure 1. Representative Standard Curve Of Human IL-6
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 2.81 - 180 pg/mL. The lower limit of detection (LLD) of this assay is 0.58 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 97 - 117% or 72 - 78% was determined by spiking defined amounts of analyte standard into cell culture supernatant or serum samples, respectively. The intra-assay precision of this assay is 3.9% (CV). The Inter-assay precision of this assay is 6.4% (CV)
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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