For detection and measurement of human interleukin-22
The Human Interleukin 22 (IL-22) ELISA Kit is designed for the quantitative detection and measurement of human IL-22 in biological fluids such as serum, urine, and plasma. Primarily produced by activated T cells and NK cells, IL-22 binds to the heterodimeric cell surface receptors IL-10R2 and IL-22R1. Both receptors signal through components of the JAK-STAT pathway and activate MAPK and p38 pathways. IL-22 impacts non-hematopoietic epithelial cells and fibroblasts in skin, digestive, and respiratory tissues. As an effector cytokine of Th17 lineage, IL-22 regulates genes associated with innate immunity of the skin. IL-22 produces antimicrobial defenses of the epidermis by inducing a range of antimicrobial proteins, as well as increasing the mobility of keratinocytes.
The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human IL-22. The captured IL-22 is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human IL-22 in the sample.
Subtype
Complete Kits
Cell Type
NK Cells, T Cells
Species
Human
Application
ELISA
Area of Interest
Immunology, Infectious Diseases
Figure 1. Representative Standard Curve Of Human IL-22
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 4.69 - 300 pg/mL. The lower limit of detection (LLD) of this assay is 0.59 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 88 - 92%, 97 - 100%, or 81 - 85% was determined by spiking defined amounts of analyte standard into serum, urine, or plasma samples, respectively, in repeated experiments. The intra-assay precision of this assay is 3.0% (CV). The Inter-assay precision of this assay is 3.7% (CV).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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