The Human Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of human IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype Complete Kits
Cell Type B Cells, Dendritic Cells, Granulocytes and Subsets, Macrophages, Monocytes
Species Human
Application ELISA
Area of Interest Immunology
Data Figures
Representative Standard Curve
• Reportable range: 3.16 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. • Sensitivity: The limit of detection of this assay is 1.53 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 95/544. • Recovery: A mid-curve recovery of 109 - 128% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 1.5% (CV). The inter-assay precision of this assay is 8.4% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
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